LOCUS       BBa_S01287              2428 bp    DNA     linear       27-Apr-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01287
VERSION     BBa_S01287.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             23..639
                     /label=tetR
     misc_feature    640..673
                     /label=SsrA
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             20..679
                     /label=BBa_C0040
     stem_loop       724..767
                     /label=stem_loop
     misc_feature    713..792
                     /label=BBa_B0010
     terminator      713..792
                     /label=BBa_B0010
     stem_loop       808..827
                     /label=T7 TE
     misc_feature    834..834
                     /label=stop
     polyA_site      828..841
                     /label=polya
     misc_feature    801..841
                     /label=BBa_B0012
     terminator      801..841
                     /label=BBa_B0012
     misc_feature    850..868
                     /label=TetR 1
     misc_feature    850..903
                     /label=BBa_R0040
     promoter        869..874
                     /label=-35
     misc_feature    875..893
                     /label=TetR 2
     promoter        892..897
                     /label=-10
     promoter        850..903
                     /label=BBa_R0040
     RBS             912..924
                     /label=RBS-3\Weak
     RBS             918..921
                     /label=RBS
     misc_feature    912..924
                     /label=BBa_B0032
     RBS             912..924
                     /label=BBa_B0032
     misc_feature    2020..2058
                     /label=LVA
     CDS             931..2058
                     /label=lacI-LVA
     misc_feature    931..2058
                     /label=BBa_C0012
     misc_feature    2059..2083
                     /label=Help:Barcodes
     CDS             931..2058
                     /label=BBa_C0012
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2092..2171
                     /label=BBa_B0010
     terminator      2092..2171
                     /label=BBa_B0010
     stem_loop       2187..2206
                     /label=T7 TE
     misc_feature    2213..2213
                     /label=stop
     polyA_site      2207..2220
                     /label=polya
     misc_feature    2180..2220
                     /label=BBa_B0012
     terminator      2180..2220
                     /label=BBa_B0012
     CDS             2229..2316
                     /label=end of LacI coding region (inactive)
     misc_feature    2229..2428
                     /label=BBa_R0010
     misc_feature    2317..2354
                     /label=CAP binding site
     promoter        2365..2370
                     /label=-35
     promoter        2389..2394
                     /label=-10
     misc_feature    2394..2428
                     /label=LacI binding site
     misc_feature    2401..2401
                     /label=start
     promoter        2229..2428
                     /label=BBa_R0010
ORIGIN
        1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat
      721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      841 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
      901 cactactaga gtcacacagg aaagtactag atggtgaatg tgaaaccagt aacgttatac
      961 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
     1021 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
     1081 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
     1141 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
     1201 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
     1261 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     1321 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     1381 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     1441 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     1501 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     1561 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     1621 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     1681 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
     1741 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     1801 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     1861 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     1921 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac
     2041 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2221 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2281 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2341 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2401 aattgtgagc ggataacaat ttcacaca
//