LOCUS BBa_S01300 4074 bp DNA linear 03-May-2024 DEFINITION Intermediate part from assembly 236 ACCESSION BBa_S01300 VERSION BBa_S01300.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 RBS 106..120 /label=RBS-1\Strong RBS 113..117 /label=RBS misc_feature 106..120 /label=BBa_B0030 RBS 106..120 /label=BBa_B0030 misc_feature 706..737 /label=LVA CDS 127..705 /label=luxI misc_feature 127..744 /label=BBa_C0061 misc_feature 745..769 /label=Help:Barcodes CDS 127..744 /label=BBa_C0061 stem_loop 789..832 /label=stem_loop misc_feature 778..857 /label=BBa_B0010 terminator 778..857 /label=BBa_B0010 stem_loop 873..892 /label=T7 TE misc_feature 899..899 /label=stop polyA_site 893..906 /label=polya misc_feature 866..906 /label=BBa_B0012 terminator 866..906 /label=BBa_B0012 CDS 915..1002 /label=end of LacI coding region (inactive) misc_feature 915..1114 /label=BBa_R0010 misc_feature 1003..1040 /label=CAP binding site promoter 1051..1056 /label=-35 promoter 1075..1080 /label=-10 misc_feature 1080..1114 /label=LacI binding site misc_feature 1087..1087 /label=start promoter 915..1114 /label=BBa_R0010 RBS 1123..1137 /label=RBS-1\Strong RBS 1130..1134 /label=RBS misc_feature 1123..1137 /label=BBa_B0030 RBS 1123..1137 /label=BBa_B0030 misc_feature 1144..1146 /label=start misc_feature 1927..1932 /label=2 misc_feature 1894..1926 /label=LVA CDS 1144..1893 /label=aiiA misc_feature 1144..1932 /label=BBa_C0060 misc_feature 1933..1957 /label=Help:Barcodes CDS 1144..1932 /label=BBa_C0060 stem_loop 1977..2020 /label=stem_loop misc_feature 1966..2045 /label=BBa_B0010 terminator 1966..2045 /label=BBa_B0010 stem_loop 2061..2080 /label=T7 TE misc_feature 2087..2087 /label=stop polyA_site 2081..2094 /label=polya misc_feature 2054..2094 /label=BBa_B0012 terminator 2054..2094 /label=BBa_B0012 misc_feature 2103..2121 /label=TetR 1 misc_feature 2103..2156 /label=BBa_R0040 promoter 2122..2127 /label=-35 misc_feature 2128..2146 /label=TetR 2 promoter 2145..2150 /label=-10 promoter 2103..2156 /label=BBa_R0040 RBS 2165..2179 /label=RBS-1\Strong RBS 2172..2176 /label=RBS misc_feature 2165..2179 /label=BBa_B0030 RBS 2165..2179 /label=BBa_B0030 CDS 2186..2947 /label=CFP (LVA) misc_feature 2904..2941 /label=SsrA misc_feature 2942..2947 /label=2 misc_feature 2254..2254 /label=A misc_feature 2186..2947 /label=BBa_E0022 misc_feature 2186..2947 /label=BBa_E0022 stem_loop 2967..3010 /label=stem_loop misc_feature 2956..3035 /label=BBa_B0010 terminator 2956..3035 /label=BBa_B0010 stem_loop 3051..3070 /label=T7 TE misc_feature 3077..3077 /label=stop polyA_site 3071..3084 /label=polya misc_feature 3044..3084 /label=BBa_B0012 terminator 3044..3084 /label=BBa_B0012 misc_feature 3093..3111 /label=TetR 1 misc_feature 3093..3146 /label=BBa_R0040 promoter 3112..3117 /label=-35 misc_feature 3118..3136 /label=TetR 2 promoter 3135..3140 /label=-10 promoter 3093..3146 /label=BBa_R0040 RBS 3155..3169 /label=RBS-1\Strong RBS 3162..3166 /label=RBS misc_feature 3155..3169 /label=BBa_B0030 RBS 3155..3169 /label=BBa_B0030 CDS 3176..3937 /label=YFP (LVA) misc_feature 3932..3937 /label=2 misc_feature 3893..3931 /label=SsrA misc_feature 3417..3417 /label=A (Q->L) misc_feature 3176..3937 /label=BBa_E0032 misc_feature 3176..3937 /label=BBa_E0032 stem_loop 3957..4000 /label=stem_loop misc_feature 3946..4025 /label=BBa_B0010 terminator 3946..4025 /label=BBa_B0010 stem_loop 4041..4060 /label=T7 TE misc_feature 4067..4067 /label=stop polyA_site 4061..4074 /label=polya misc_feature 4034..4074 /label=BBa_B0012 terminator 4034..4074 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagattaa agaggagaaa 121 tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat 181 aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt 241 gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct 301 tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat 361 atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat 421 atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct 481 agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt 541 acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt 601 ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta 661 ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc aaacgacgaa 721 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 901 tttatatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 961 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 1021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 1081 gtgtggaatt gtgagcggat aacaatttca cacatactag agattaaaga ggagaaatac 1141 tagatgacag taaagaagct ttatttcgtc ccagcaggtc gttgtatgtt ggatcattcg 1201 tctgttaata gtacattaac accaggagaa ttattagact taccggtttg gtgttatctt 1261 ttggagactg aagaaggacc tattttagta gatacaggta tgccagaaag tgcagttaat 1321 aatgaaggtc tttttaacgg tacatttgtc gaagggcagg ttttaccgaa aatgactgaa 1381 gaagatagaa tcgtgaatat tttaaaacgg gttggttatg agccggaaga ccttctttat 1441 attattagtt ctcacttgca ttttgatcat gcaggaggaa atggcgcttt tataaataca 1501 ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc atagcgaaga atatttgaaa 1561 gaatgtatat tgccgaattt aaactacaaa atcattgaag gtgattatga agtcgtacca 1621 ggagttcaat tattgcatac accaggccat actccagggc atcaatcgct attaattgag 1681 acagaaaaat ccggtcctgt attattaacg attgatgcat cgtatacgaa agagaatttt 1741 gaaaatgaag tgccatttgc gggatttgat tcagaattag ctttatcttc aattaaacgt 1801 ttaaaagaag tggtgatgaa agagaagccg attgttttct ttggacatga tatagagcag 1861 gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa acgacgaaaa ctacgcttta 1921 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa 1981 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2041 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2101 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact 2161 agagattaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt 2221 ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg 2281 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 2341 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt 2401 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 2461 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 2521 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 2581 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta 2641 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat 2701 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 2761 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 2821 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 2881 cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc 2941 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3001 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3061 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc 3121 ctatcagtga tagagatact gagcactact agagattaaa gaggagaaat actagatggt 3181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 3241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 3301 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 3361 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca 3421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 3481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 3541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 3601 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 3661 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 3721 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 3781 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 3841 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaggcctgc 3901 tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa 3961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 4021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //