LOCUS       BBa_S01374              1956 bp    DNA     linear       23-May-2024
DEFINITION  Intermediate part from assembly 239
ACCESSION   BBa_S01374
VERSION     BBa_S01374.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=start
     misc_feature    802..807
                     /label=2
     misc_feature    769..801
                     /label=LVA
     CDS             19..768
                     /label=aiiA
     misc_feature    19..807
                     /label=BBa_C0060
     misc_feature    808..832
                     /label=Help:Barcodes
     CDS             19..807
                     /label=BBa_C0060
     stem_loop       852..895
                     /label=stem_loop
     misc_feature    841..920
                     /label=BBa_B0010
     terminator      841..920
                     /label=BBa_B0010
     stem_loop       936..955
                     /label=T7 TE
     misc_feature    962..962
                     /label=stop
     polyA_site      956..969
                     /label=polya
     misc_feature    929..969
                     /label=BBa_B0012
     terminator      929..969
                     /label=BBa_B0012
     misc_feature    978..996
                     /label=TetR 1
     misc_feature    978..1031
                     /label=BBa_R0040
     promoter        997..1002
                     /label=-35
     misc_feature    1003..1021
                     /label=TetR 2
     promoter        1020..1025
                     /label=-10
     promoter        978..1031
                     /label=BBa_R0040
     misc_feature    1044..1047
                     /label=conserved
     RBS             1040..1051
                     /label=BBa_B0034
     CDS             1058..1819
                     /label=YFP (LVA)
     misc_feature    1814..1819
                     /label=2
     misc_feature    1775..1813
                     /label=SsrA
     misc_feature    1299..1299
                     /label=A (Q->L)
     misc_feature    1058..1819
                     /label=BBa_E0032
     misc_feature    1058..1819
                     /label=BBa_E0032
     stem_loop       1839..1882
                     /label=stem_loop
     misc_feature    1828..1907
                     /label=BBa_B0010
     terminator      1828..1907
                     /label=BBa_B0010
     stem_loop       1923..1942
                     /label=T7 TE
     misc_feature    1949..1949
                     /label=stop
     polyA_site      1943..1956
                     /label=polya
     misc_feature    1916..1956
                     /label=BBa_B0012
     terminator      1916..1956
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt
       61 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg
      121 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca
      181 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta
      241 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg
      301 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc
      361 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc
      421 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat
      481 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa
      541 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat
      601 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta
      661 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga
      721 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac
      781 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
     1021 atactgagca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct
     1081 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1141 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1201 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1261 cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct tcaagtccgc
     1321 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1381 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1441 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1501 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1561 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1621 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     1681 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1741 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta
     1801 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1861 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1921 ctggctcacc ttcgggtggg cctttctgcg tttata
//