LOCUS       BBa_S01451              2205 bp    DNA     linear       23-May-2024
DEFINITION  Intermediate part from assembly 243
ACCESSION   BBa_S01451
VERSION     BBa_S01451.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1975..1980
                     /label=2
     CDS             1237..1980
                     /label=cI HK022
     misc_feature    1942..1974
                     /label=SsrA
     misc_feature    1237..1244
                     /label=OR3 partial
     misc_feature    1237..1980
                     /label=BBa_C0050
     misc_feature    1981..2005
                     /label=Help:Barcodes
     CDS             1237..1980
                     /label=BBa_C0050
     stem_loop       2025..2068
                     /label=stem_loop
     misc_feature    2014..2093
                     /label=BBa_B0010
     terminator      2014..2093
                     /label=BBa_B0010
     stem_loop       2109..2128
                     /label=T7 TE
     misc_feature    2135..2135
                     /label=stop
     polyA_site      2129..2142
                     /label=polya
     misc_feature    2102..2142
                     /label=BBa_B0012
     terminator      2102..2142
                     /label=BBa_B0012
     promoter        2158..2163
                     /label=-35
     RBS             2163..2177
                     /label=OR2
     promoter        2180..2185
                     /label=-10
     RBS             2187..2201
                     /label=OR1
     misc_feature    2193..2193
                     /label=putative
     misc_feature    2151..2205
                     /label=BBa_R0050
     promoter        2151..2205
                     /label=BBa_R0050
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg ttcaacagaa agagcgtgaa
     1261 actttctcgc agaggcttgc gctggcctgt gataaagcgg gattaccttt gcatggtagg
     1321 caggctgatt tagctgtcag gcttaaggtc acaccaaaag ccattagtaa atggttcaac
     1381 ggggagtcaa taccaagaaa agacaagatg gaatctctgg cttcggtgct gggaactact
     1441 gctgcatatc tgcatggcta tgctgatgat gacggtatca cggtaaatca tctatcaaga
     1501 tcaaatgatt attatcgtgt tgatgtattg gatgttcagg cgagcgccgg gccaggaacc
     1561 atggtttcca atgaatttat agaaaagata agagcaattg aatatacgac cgagcaggca
     1621 agaattttat ttaatggaag gccacaggaa agcgtaaaag tcatcacggt tcgcggtgac
     1681 agcatggagg gaaccatcaa tccgggagat gagatctttg ttgatgtatc cataacctgt
     1741 tttgatggcg atggcattta tgtgtttgta tacgggaaaa caatgcacgt taagcgcctg
     1801 caaatgcaaa agaacaggct tgccgtcatc tctgacaatg ccgcttatga tcgatggtac
     1861 atagaagaag gtgaagaaga gcaacttcac attctagcca aagtcctcat taggcagtca
     1921 atcgattaca agcgattcgg agctgcaaac gacgaaaact acgctttagt agcttaataa
     1981 ccctgatagt gctagtgtag atccctacta gagccaggca tcaaataaaa cgaaaggctc
     2041 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2101 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttaggtattg
     2161 actgtactat cagttccgtc ataatatgaa ccataagttc accac
//