LOCUS       BBa_S01476               249 bp    DNA     linear       17-May-2024
DEFINITION  Intermediate part from assembly 233
ACCESSION   BBa_S01476
VERSION     BBa_S01476.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     CDS             50..137
                     /label=end of LacI coding region (inactive)
     misc_feature    50..249
                     /label=BBa_R0010
     misc_feature    138..175
                     /label=CAP binding site
     promoter        186..191
                     /label=-35
     promoter        210..215
                     /label=-10
     misc_feature    215..249
                     /label=LacI binding site
     misc_feature    222..222
                     /label=start
     promoter        50..249
                     /label=BBa_R0010
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa
       61 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      121 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      181 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      241 tttcacaca
//