LOCUS       BBa_S01675              1827 bp    DNA     linear       08-May-2024
DEFINITION  Q04400.E0432
ACCESSION   BBa_S01675
VERSION     BBa_S01675.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     CDS             929..1690
                     /label=YFP (LVA)
     misc_feature    1685..1690
                     /label=2
     misc_feature    1646..1684
                     /label=SsrA
     misc_feature    1170..1170
                     /label=A (Q->L)
     misc_feature    929..1690
                     /label=BBa_E0032
     misc_feature    929..1690
                     /label=BBa_E0032
     stem_loop       1710..1753
                     /label=stem_loop
     misc_feature    1699..1778
                     /label=BBa_B0010
     terminator      1699..1778
                     /label=BBa_B0010
     stem_loop       1794..1813
                     /label=T7 TE
     misc_feature    1820..1820
                     /label=stop
     polyA_site      1814..1827
                     /label=polya
     misc_feature    1787..1827
                     /label=BBa_B0012
     terminator      1787..1827
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg
      961 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     1021 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     1081 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg
     1141 cttcgcccgc taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga
     1201 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1261 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1321 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1381 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1441 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1501 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     1561 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1621 tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt
     1681 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1741 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1801 cttcgggtgg gcctttctgc gtttata
//