LOCUS BBa_S01840 4795 bp DNA linear 29-Apr-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01840 VERSION BBa_S01840.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 promoter 1038..1043 /label=-10 promoter 1015..1020 /label=-35 misc_feature 1025..1041 /label=OR1 misc_feature 1001..1017 /label=OR2 misc_feature 1001..1049 /label=BBa_R0051 promoter 1001..1049 /label=BBa_R0051 misc_feature 1062..1065 /label=conserved RBS 1058..1069 /label=BBa_B0034 CDS 1076..1723 /label=cII p22 misc_feature 1724..1756 /label=LVA misc_feature 1757..1762 /label=stop misc_feature 1076..1762 /label=BBa_C0053 misc_feature 1763..1787 /label=Help:Barcodes CDS 1076..1762 /label=BBa_C0053 stem_loop 1807..1850 /label=stem_loop misc_feature 1796..1875 /label=BBa_B0010 terminator 1796..1875 /label=BBa_B0010 stem_loop 1891..1910 /label=T7 TE misc_feature 1917..1917 /label=stop polyA_site 1911..1924 /label=polya misc_feature 1884..1924 /label=BBa_B0012 terminator 1884..1924 /label=BBa_B0012 misc_feature 1933..1935 /label=OR3 misc_feature 1943..1960 /label=OR2 misc_feature 1966..1983 /label=OR1 promoter 1950..1955 /label=-35 promoter 1940..1945 /label=-35 promoter 1962..1967 /label=-10 misc_feature 1933..1986 /label=BBa_R0053 promoter 1933..1986 /label=BBa_R0053 misc_feature 1999..2002 /label=conserved RBS 1995..2006 /label=BBa_B0034 CDS 2016..2632 /label=tetR misc_feature 2633..2666 /label=SsrA misc_feature 2673..2697 /label=Help:Barcodes CDS 2013..2672 /label=BBa_C0040 stem_loop 2717..2760 /label=stem_loop misc_feature 2706..2785 /label=BBa_B0010 terminator 2706..2785 /label=BBa_B0010 stem_loop 2801..2820 /label=T7 TE misc_feature 2827..2827 /label=stop polyA_site 2821..2834 /label=polya misc_feature 2794..2834 /label=BBa_B0012 terminator 2794..2834 /label=BBa_B0012 misc_feature 2843..2861 /label=TetR 1 misc_feature 2843..2896 /label=BBa_R0040 promoter 2862..2867 /label=-35 misc_feature 2868..2886 /label=TetR 2 promoter 2885..2890 /label=-10 promoter 2843..2896 /label=BBa_R0040 misc_feature 2909..2912 /label=conserved RBS 2905..2916 /label=BBa_B0034 CDS 2923..3684 /label=CFP (LVA) misc_feature 3641..3678 /label=SsrA misc_feature 3679..3684 /label=2 misc_feature 2991..2991 /label=A misc_feature 2923..3684 /label=BBa_E0022 misc_feature 2923..3684 /label=BBa_E0022 stem_loop 3704..3747 /label=stem_loop misc_feature 3693..3772 /label=BBa_B0010 terminator 3693..3772 /label=BBa_B0010 stem_loop 3788..3807 /label=T7 TE misc_feature 3814..3814 /label=stop polyA_site 3808..3821 /label=polya misc_feature 3781..3821 /label=BBa_B0012 terminator 3781..3821 /label=BBa_B0012 misc_feature 1..3821 /label=BBa_I5600 misc_feature 3832..3848 /label=lac O1 promoter 3849..3854 /label=-35 misc_feature 3855..3871 /label=lac O1 promoter 3872..3877 /label=-10 misc_feature 3830..3883 /label=BBa_R0011 promoter 3830..3883 /label=BBa_R0011 misc_feature 3897..3900 /label=conserved RBS 3893..3904 /label=BBa_B0034 CDS 3911..4579 /label=cI 434 misc_feature 4541..4579 /label=LVA misc_feature 3911..4579 /label=BBa_C0052 misc_feature 4580..4604 /label=Help:Barcodes CDS 3911..4579 /label=BBa_C0052 stem_loop 4624..4667 /label=stem_loop misc_feature 4613..4692 /label=BBa_B0010 terminator 4613..4692 /label=BBa_B0010 stem_loop 4708..4727 /label=T7 TE misc_feature 4734..4734 /label=stop polyA_site 4728..4741 /label=polya misc_feature 4701..4741 /label=BBa_B0012 terminator 4701..4741 /label=BBa_B0012 terminator 4613..4741 /label=BBa_B0015 misc_feature 4750..4792 /label=OR2 misc_feature 4757..4770 /label=OR2 misc_feature 4779..4792 /label=OR1 promoter 4750..4755 /label=-35 promoter 4773..4778 /label=-10 promoter 4751..4756 /label=-35 misc_feature 4784..4786 /label=start misc_feature 4750..4795 /label=BBa_R0052 promoter 4750..4795 /label=BBa_R0052 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact 1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatgaa 1081 tacacaattg atgggtgagc gtattcgcgc tcgaagaaaa aaactcaaga ttagacaagc 1141 cgctcttggt aagatggtgg gagtgtctaa tgttgcaata tcgcaatggg agcgctcgga 1201 gactgagcca aatggggaga acctgttggc actttcgaag gctcttcagt gctcccctga 1261 ctatttgctg aaaggagatt taagccagac aaacgttgcc tatcatagta ggcatgagcc 1321 aagaggatca taccctctta tcagttgggt aagcgcaggg caatggatgg aagctgtaga 1381 accttatcac aagcgcgcga tagagaactg gcacgacacc actgtagatt gttcagaaga 1441 ttcattttgg cttgatgtcc aaggtgactc tatgacagca ccggcagggt taagcattcc 1501 agaaggaatg ataattctgg ttgatcccga agtcgaacca agaaacggca agctggttgt 1561 tgcaaaatta gaaggtgaaa acgaggccac attcaaaaaa ttagttatgg atgcaggccg 1621 aaagttttta aaaccattaa acccacaata tccgatgata gaaatcaacg gaaactgcaa 1681 aatcattggc gtagttgttg acgcaaaact cgcaaatctt ccagctgcaa acgacgaaaa 1741 ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg 1801 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1861 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 1921 tatatactag agaataaact tgactaaaga ttcctttagt agataattta agtgttcttt 1981 aatttctact agagaaagag gagaaatact agatgtccag attagataaa agtaaagtga 2041 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac 2101 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg 2161 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt 2221 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt 2281 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt 2341 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg 2401 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag 2461 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat 2521 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc 2581 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa 2641 acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac 2701 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2761 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2821 tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga 2881 tagagatact gagcactact agagaaagag gagaaatact agatggtgag caagggcgag 2941 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac 3001 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 3061 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 3121 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 3181 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 3241 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 3301 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 3361 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 3421 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 3481 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 3541 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 3601 gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctgctgc aaacgacgaa 3661 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca 3721 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 3781 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg 3841 gataacaatt gacattgtga gcggataaca agatactgag cacatactag agaaagagga 3901 gaaatactag atgagtattt cttccagggt aaaaagcaaa agaatccagc ttggacttaa 3961 ccaggctgaa cttgctcaaa aggtggggac tacccagcag tctatagagc agctcgaaaa 4021 cggtaaaact aagcgaccac gctttttacc agaacttgcg tcagctcttg gcgtaagtgt 4081 tgactggctg ctcaatggca cctctgattc gaatgttaga tttgttgggc acgttgagcc 4141 caaagggaaa tatccattga ttagcatggt tagagctggt tcgtggtgtg aagcttgtga 4201 accctacgat atcaaggaca ttgatgaatg gtatgacagt gacgttaact tattaggcaa 4261 tggattctgg ctgaaggttg aaggtgattc catgacctca cctgtaggtc aaagcatccc 4321 tgaaggtcat atggtgttag tagatactgg acgggagcca gtgaatggaa gccttgttgt 4381 agccaaactg actgacgcga acgaagcaac attcaagaaa ctggtcatag atggcggtca 4441 gaagtacctg aaaggcctga atccttcatg gcctatgact cctatcaacg gaaactgcaa 4501 gattatcggt gttgtcgtgg aagcgagggt aaaattcgta gctgcaaacg acgaaaacta 4561 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 4621 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4681 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4741 atactagagt tgacaaacaa gatacattgt atgaaaatac aagaaagttt gttga //