LOCUS BBa_S01849 5441 bp DNA linear 03-May-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01849 VERSION BBa_S01849.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..741 /label=BBa_C0040 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 2081..2119 /label=LVA CDS 992..2119 /label=lacI-LVA misc_feature 992..2119 /label=BBa_C0012 misc_feature 2120..2144 /label=Help:Barcodes CDS 992..2119 /label=BBa_C0012 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 CDS 2290..2377 /label=end of LacI coding region (inactive) misc_feature 2290..2489 /label=BBa_R0010 misc_feature 2378..2415 /label=CAP binding site promoter 2426..2431 /label=-35 promoter 2450..2455 /label=-10 misc_feature 2455..2489 /label=LacI binding site misc_feature 2462..2462 /label=start promoter 2290..2489 /label=BBa_R0010 misc_feature 2502..2505 /label=conserved RBS 2498..2509 /label=BBa_B0034 CDS 2519..3226 /label=cI lambda misc_feature 3227..3259 /label=LVA misc_feature 3266..3290 /label=Help:Barcodes CDS 2516..3265 /label=BBa_C0051 stem_loop 3310..3353 /label=stem_loop misc_feature 3299..3378 /label=BBa_B0010 terminator 3299..3378 /label=BBa_B0010 stem_loop 3394..3413 /label=T7 TE misc_feature 3420..3420 /label=stop polyA_site 3414..3427 /label=polya misc_feature 3387..3427 /label=BBa_B0012 terminator 3387..3427 /label=BBa_B0012 misc_feature 3436..3532 /label=BBa_R0065 misc_feature 3441..3460 /label=Lux Box promoter 3482..3487 /label=-10 misc_feature 3492..3508 /label=OR2 cI promoter 3493..3493 /label=lux p(R) start promoter 3506..3511 /label=-35 misc_feature 3516..3532 /label=OR1 cI promoter 3529..3532 /label=-10 promoter 3436..3532 /label=BBa_R0065 misc_feature 3545..3548 /label=conserved RBS 3541..3552 /label=BBa_B0034 misc_feature 4138..4169 /label=LVA CDS 3559..4137 /label=luxI misc_feature 3559..4176 /label=BBa_C0061 misc_feature 4177..4201 /label=Help:Barcodes CDS 3559..4176 /label=BBa_C0061 stem_loop 4221..4264 /label=stem_loop misc_feature 4210..4289 /label=BBa_B0010 terminator 4210..4289 /label=BBa_B0010 stem_loop 4305..4324 /label=T7 TE misc_feature 4331..4331 /label=stop polyA_site 4325..4338 /label=polya misc_feature 4298..4338 /label=BBa_B0012 terminator 4298..4338 /label=BBa_B0012 misc_feature 4347..4366 /label=LuxR/HSL promoter 4461..4468 /label=-10 promoter 4435..4440 /label=-35 misc_feature 4474..4474 /label=start misc_feature 4476..4495 /label=Putative LuxR/HSL misc_feature 4347..4497 /label=BBa_R0063 promoter 4347..4497 /label=BBa_R0063 misc_feature 4510..4513 /label=conserved RBS 4506..4517 /label=BBa_B0034 misc_feature 4524..4525 /label=prefix misc_feature 4697..4697 /label=T misc_feature 5015..5015 /label=A CDS 4524..5273 /label=luxR misc_feature 4524..5279 /label=BBa_C0062 misc_feature 5280..5304 /label=Help:Barcodes CDS 4524..5279 /label=BBa_C0062 stem_loop 5324..5367 /label=stem_loop misc_feature 5313..5392 /label=BBa_B0010 terminator 5313..5392 /label=BBa_B0010 stem_loop 5408..5427 /label=T7 TE misc_feature 5434..5434 /label=stop polyA_site 5428..5441 /label=polya misc_feature 5401..5441 /label=BBa_B0012 terminator 5401..5441 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata 1021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 1081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 1141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 1201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 2041 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 2341 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 2401 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 2461 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatgag 2521 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 2581 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 2641 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 2701 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 2761 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 2821 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt 2881 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 2941 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 3001 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 3061 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 3121 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 3181 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga 3241 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 3301 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3361 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3421 gtttatatac tagagtaagc acctgtagga tcgtacaggt ttacgcaaga aaatggtttg 3481 ttatagtcga ataacaccgt gcgtgttgac tattttacct ctggcggtga tatactagag 3541 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 3601 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 3661 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 3721 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 3781 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 3841 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 3901 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 3961 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 4021 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 4081 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 4141 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 4201 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 4261 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 4321 ggcctttctg cgtttatata ctagagacct gtacgatcct acaggtgctt atgttaagta 4381 attgtattcc cagcgataca atagtgtgac aaaaatccaa tttattagaa tcaaatgtca 4441 atccattacc gttttaatga tatataacac gcaaaacttg cgacaaacaa taggtaatac 4501 tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa 4561 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga 4621 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta 4681 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg 4741 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta 4801 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag 4861 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct 4921 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac 4981 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa 5041 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt 5101 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta 5161 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa 5221 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac 5281 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 5341 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 5401 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //