LOCUS BBa_S01851 3337 bp DNA linear 01-May-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01851 VERSION BBa_S01851.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 promoter 1038..1043 /label=-10 promoter 1015..1020 /label=-35 misc_feature 1025..1041 /label=OR1 misc_feature 1001..1017 /label=OR2 misc_feature 1001..1049 /label=BBa_R0051 promoter 1001..1049 /label=BBa_R0051 misc_feature 1062..1065 /label=conserved RBS 1058..1069 /label=BBa_B0034 misc_feature 2165..2203 /label=LVA CDS 1076..2203 /label=lacI-LVA misc_feature 1076..2203 /label=BBa_C0012 misc_feature 2204..2228 /label=Help:Barcodes CDS 1076..2203 /label=BBa_C0012 stem_loop 2248..2291 /label=stem_loop misc_feature 2237..2316 /label=BBa_B0010 terminator 2237..2316 /label=BBa_B0010 stem_loop 2332..2351 /label=T7 TE misc_feature 2358..2358 /label=stop polyA_site 2352..2365 /label=polya misc_feature 2325..2365 /label=BBa_B0012 terminator 2325..2365 /label=BBa_B0012 misc_feature 2376..2392 /label=lac O1 promoter 2393..2398 /label=-35 misc_feature 2399..2415 /label=lac O1 promoter 2416..2421 /label=-10 misc_feature 2374..2427 /label=BBa_R0011 promoter 2374..2427 /label=BBa_R0011 misc_feature 2440..2443 /label=conserved RBS 2436..2447 /label=BBa_B0034 CDS 2457..3073 /label=tetR misc_feature 3074..3107 /label=SsrA misc_feature 3114..3138 /label=Help:Barcodes CDS 2454..3113 /label=BBa_C0040 stem_loop 3158..3201 /label=stem_loop misc_feature 3147..3226 /label=BBa_B0010 terminator 3147..3226 /label=BBa_B0010 stem_loop 3242..3261 /label=T7 TE misc_feature 3268..3268 /label=stop polyA_site 3262..3275 /label=polya misc_feature 3235..3275 /label=BBa_B0012 terminator 3235..3275 /label=BBa_B0012 misc_feature 3284..3302 /label=TetR 1 misc_feature 3284..3337 /label=BBa_R0040 promoter 3303..3308 /label=-35 misc_feature 3309..3327 /label=TetR 2 promoter 3326..3331 /label=-10 promoter 3284..3337 /label=BBa_R0040 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact 1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggt 1081 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac 1141 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga 1201 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa 1261 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat 1321 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt 1381 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt 1441 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc 1501 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat 1561 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca 1621 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc 1681 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga 1741 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc 1801 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga 1861 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag 1921 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 1981 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 2041 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 2101 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 2161 gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 2221 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2281 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2341 tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac aattgacatt 2401 gtgagcggat aacaagatac tgagcactac tagagaaaga ggagaaatac tagatgtcca 2461 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 2521 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 2581 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 2641 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 2701 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 2761 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 2821 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 2881 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 2941 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 3001 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 3061 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 3121 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 3181 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 3241 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga 3301 gattgacatc cctatcagtg atagagatac tgagcac //