LOCUS       BBa_S01897              2019 bp    DNA     linear       04-May-2024
DEFINITION  --Specify Parts List--
ACCESSION   BBa_S01897
VERSION     BBa_S01897.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     misc_feature    865..870
                     /label=2
     misc_feature    832..864
                     /label=LVA
     CDS             82..831
                     /label=aiiA
     misc_feature    82..870
                     /label=BBa_C0060
     misc_feature    871..895
                     /label=Help:Barcodes
     CDS             82..870
                     /label=BBa_C0060
     stem_loop       915..958
                     /label=stem_loop
     misc_feature    904..983
                     /label=BBa_B0010
     terminator      904..983
                     /label=BBa_B0010
     stem_loop       999..1018
                     /label=T7 TE
     misc_feature    1025..1025
                     /label=stop
     polyA_site      1019..1032
                     /label=polya
     misc_feature    992..1032
                     /label=BBa_B0012
     terminator      992..1032
                     /label=BBa_B0012
     misc_feature    64..1032
                     /label=BBa_I0460
     misc_feature    1041..1059
                     /label=TetR 1
     misc_feature    1041..1094
                     /label=BBa_R0040
     promoter        1060..1065
                     /label=-35
     misc_feature    1066..1084
                     /label=TetR 2
     promoter        1083..1088
                     /label=-10
     promoter        1041..1094
                     /label=BBa_R0040
     misc_feature    1107..1110
                     /label=conserved
     RBS             1103..1114
                     /label=BBa_B0034
     CDS             1121..1882
                     /label=YFP (LVA)
     misc_feature    1877..1882
                     /label=2
     misc_feature    1838..1876
                     /label=SsrA
     misc_feature    1362..1362
                     /label=A (Q->L)
     misc_feature    1121..1882
                     /label=BBa_E0032
     misc_feature    1121..1882
                     /label=BBa_E0032
     stem_loop       1902..1945
                     /label=stem_loop
     misc_feature    1891..1970
                     /label=BBa_B0010
     terminator      1891..1970
                     /label=BBa_B0010
     stem_loop       1986..2005
                     /label=T7 TE
     misc_feature    2012..2012
                     /label=stop
     polyA_site      2006..2019
                     /label=polya
     misc_feature    1979..2019
                     /label=BBa_B0012
     terminator      1979..2019
                     /label=BBa_B0012
     misc_feature    1103..2019
                     /label=BBa_E0432
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
      121 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
      181 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
      241 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
      301 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
      361 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
      421 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
      481 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
      541 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
      601 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
      661 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
      721 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
      781 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac
      841 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      901 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      961 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1021 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata
     1081 gagatactga gcactactag agaaagagga gaaatactag atggtgagca agggcgagga
     1141 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1201 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1261 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     1321 cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc
     1381 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1441 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1501 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1561 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1621 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1681 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     1741 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1801 cgccgggatc actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa
     1861 ctacgcttta gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt
     1921 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1981 acactggctc accttcgggt gggcctttct gcgtttata
//