LOCUS       BBa_S03165              2065 bp    DNA     linear       17-May-2024
DEFINITION  R0011.B0034.C0051.B0034.C0061.E0433
ACCESSION   BBa_S03165
VERSION     BBa_S03165.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..830
                     /label=BBa_C0051
     misc_feature    868..871
                     /label=conserved
     RBS             864..875
                     /label=BBa_B0034
     misc_feature    1461..1492
                     /label=LVA
     CDS             882..1460
                     /label=luxI
     misc_feature    882..1499
                     /label=BBa_C0061
     misc_feature    1500..1524
                     /label=Help:Barcodes
     CDS             882..1499
                     /label=BBa_C0061
     misc_feature    1537..1540
                     /label=conserved
     RBS             1533..1544
                     /label=BBa_B0034
     CDS             1551..1898
                     /label=lacZ alpha
     misc_feature    1631..1631
                     /label=T
     misc_feature    1637..1637
                     /label=A
     misc_feature    1658..1658
                     /label=C
     misc_feature    1628..1628
                     /label=C
     misc_feature    1661..1661
                     /label=G
     promoter        1576..1585
                     /label=T3 promoter
     promoter        1723..1741
                     /label=T7 promoter
     misc_feature    1749..1898
                     /label=lacZ gene fragment
     misc_feature    1551..1565
                     /label=lacZ gene fragment
     misc_feature    1551..1903
                     /label=BBa_E0033
     stem_loop       1948..1991
                     /label=stem_loop
     misc_feature    1937..2016
                     /label=BBa_B0010
     terminator      1937..2016
                     /label=BBa_B0010
     stem_loop       2032..2051
                     /label=T7 TE
     misc_feature    2058..2058
                     /label=stop
     polyA_site      2052..2065
                     /label=polya
     misc_feature    2025..2065
                     /label=BBa_B0012
     terminator      2025..2065
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagaaagagg agaaatacta gatgactata atgataaaaa
      901 aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc
      961 aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg
     1021 agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg
     1081 gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat
     1141 tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg
     1201 taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg
     1261 aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa
     1321 cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag
     1381 aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt
     1441 ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1501 tctgatagtg ctagtgtaga tctctactag agaaagagga gaaatactag atgaccatga
     1561 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg
     1621 tggcggcagc actagagcta gtggatcccc cgggctgtag aaattcgata tcaagcttat
     1681 cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
     1741 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1801 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1861 gcaccgatcg cccttcccaa cagttgcgca gcctgaataa taacgctgat agtgctagtg
     1921 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1981 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2041 tcgggtgggc ctttctgcgt ttata
//