LOCUS       BBa_S03168              1708 bp    DNA     linear       17-May-2024
DEFINITION  Q04400.I0461
ACCESSION   BBa_S03168
VERSION     BBa_S03168.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     misc_feature    1508..1539
                     /label=LVA
     CDS             929..1507
                     /label=luxI
     misc_feature    929..1546
                     /label=BBa_C0061
     misc_feature    1547..1571
                     /label=Help:Barcodes
     CDS             929..1546
                     /label=BBa_C0061
     stem_loop       1591..1634
                     /label=stem_loop
     misc_feature    1580..1659
                     /label=BBa_B0010
     terminator      1580..1659
                     /label=BBa_B0010
     stem_loop       1675..1694
                     /label=T7 TE
     misc_feature    1701..1701
                     /label=stop
     polyA_site      1695..1708
                     /label=polya
     misc_feature    1668..1708
                     /label=BBa_B0012
     terminator      1668..1708
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt
      961 ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca
     1021 aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc
     1081 aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt
     1141 attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca
     1201 gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag
     1261 ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa
     1321 acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg
     1381 atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt
     1441 aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt
     1501 cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta
     1561 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1621 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1681 ccttcgggtg ggcctttctg cgtttata
//