LOCUS       BBa_S03337               951 bp    DNA     linear       10-May-2024
DEFINITION  Prm+ with RFP as a reporter
ACCESSION   BBa_S03337
VERSION     BBa_S03337.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..783
                     /label=mrfp1
     misc_feature    790..814
                     /label=Help:Barcodes
     CDS             109..789
                     /label=BBa_E1010
     stem_loop       834..877
                     /label=stem_loop
     misc_feature    823..902
                     /label=BBa_B0010
     terminator      823..902
                     /label=BBa_B0010
     stem_loop       918..937
                     /label=T7 TE
     misc_feature    944..944
                     /label=stop
     polyA_site      938..951
                     /label=polya
     misc_feature    911..951
                     /label=BBa_B0012
     terminator      911..951
                     /label=BBa_B0012
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat ggcttcctcc
      121 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      181 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      241 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      301 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      361 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
      421 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      481 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
      541 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
      601 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
      661 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
      721 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
      781 gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac
      841 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      901 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//