LOCUS       BBa_S03338              1891 bp    DNA     linear       13-May-2024
DEFINITION  Pr with GFP and Prm+ with RFP
ACCESSION   BBa_S03338
VERSION     BBa_S03338.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
     misc_feature    949..965
                     /label=OR1
     misc_feature    973..989
                     /label=OR2
     promoter        988..993
                     /label=-35
     misc_feature    999..1005
                     /label=mutate to obliterate OR3
     promoter        1011..1016
                     /label=-10
     promoter        941..1022
                     /label=BBa_I12007
     misc_feature    1035..1038
                     /label=conserved
     RBS             1031..1042
                     /label=BBa_B0034
     CDS             1049..1723
                     /label=mrfp1
     misc_feature    1730..1754
                     /label=Help:Barcodes
     CDS             1049..1729
                     /label=BBa_E1010
     stem_loop       1774..1817
                     /label=stem_loop
     misc_feature    1763..1842
                     /label=BBa_B0010
     terminator      1763..1842
                     /label=BBa_B0010
     stem_loop       1858..1877
                     /label=T7 TE
     misc_feature    1884..1884
                     /label=stop
     polyA_site      1878..1891
                     /label=polya
     misc_feature    1851..1891
                     /label=BBa_B0012
     terminator      1851..1891
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag gcaaccatta tcaccgccag
      961 aggtaaaata gtcaacacgc acggtgttag atatttataa atagtggtga tagatttaac
     1021 gttactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt
     1081 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg
     1141 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg
     1201 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc
     1261 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt
     1321 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc
     1381 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc
     1441 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta
     1501 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg
     1561 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg
     1621 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
     1681 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg
     1741 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1801 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1861 tcaccttcgg gtgggccttt ctgcgtttat a
//