LOCUS       BBa_S03416              1907 bp    DNA     linear       29-Apr-2024
DEFINITION  S03414.S03155
ACCESSION   BBa_S03416
VERSION     BBa_S03416.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    883..907
                     /label=Help:Barcodes
     CDS             157..882
                     /label=BBa_I15008
     misc_feature    920..923
                     /label=conserved
     RBS             916..927
                     /label=BBa_B0034
     CDS             934..1683
                     /label=PcyA
     misc_feature    1684..1708
                     /label=Help:Barcodes
     CDS             934..1683
                     /label=BBa_I15009
     stem_loop       1728..1771
                     /label=stem_loop
     misc_feature    1717..1796
                     /label=BBa_B0010
     terminator      1717..1796
                     /label=BBa_B0010
     stem_loop       1812..1831
                     /label=T7 TE
     misc_feature    1838..1838
                     /label=stop
     polyA_site      1832..1845
                     /label=polya
     misc_feature    1805..1845
                     /label=BBa_B0012
     terminator      1805..1845
                     /label=BBa_B0012
     misc_feature    1854..1872
                     /label=TetR 1
     misc_feature    1854..1907
                     /label=BBa_R0040
     promoter        1873..1878
                     /label=-35
     misc_feature    1879..1897
                     /label=TetR 2
     promoter        1896..1901
                     /label=-10
     promoter        1854..1907
                     /label=BBa_R0040
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga gtgtcaactt agcttcccag
      181 ttgcgggaag ggacgaaaaa atcccactcc atggcggaga acgtcggctt tgtcaaatgc
      241 ttcctcaagg gcgttgtcga gaaaaattcc taccgtaagc tggttggcaa tctctacttt
      301 gtctacagtg ccatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac
      361 atttacttcc ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac
      421 ggctccaact ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga
      481 gtccggcaag tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac
      541 ctgggggatc tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc
      601 cacgatggtg gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa
      661 aatacctacc gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt
      721 gtggatgaag ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc
      781 aacctgatca aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa
      841 ggcagcaccg aagttggcct cgccacctcc gaaggctaat aacgctgata gtgctagtgt
      901 agatcgctac tagagaaaga ggagaaatac tagatggccg tcactgattt aagtttgacc
      961 aattcttccc tgatgcctac gttgaacccg atgattcaac agttggccct ggcgatcgcc
     1021 gctagttggc aaagtttacc cctcaagccc tatcaattgc cggaggattt gggctacgta
     1081 gaaggccgcc tggaagggga aaagttagtg attgaaaatc ggtgctacca aacgccccag
     1141 tttcgcaaaa tgcatttgga gttggccaag gtgggcaaag ggttggatat tctccactgt
     1201 gtaatgtttc ctgagccttt atacggtcta cctttgtttg gctgtgacat tgtggccggc
     1261 cccggtggag taagtgcggc tattgcggat ctatccccca cccaaagcga tcgccaattg
     1321 cccgcagcgt accaaaaatc attggcagag ctaggccagc cagaatttga gcaacaacgg
     1381 gaattgcccc cctggggaga aatattttct gaatattgtt tattcatccg tcccagcaat
     1441 gtcactgaag aagaaagatt tgtacaaagg gtagtggact ttttgcaaat tcattgtcac
     1501 caatccatcg ttgccgaacc cttgtctgaa gctcaaactt tggagcaccg tcaggggcaa
     1561 attcattact gccaacaaca acagaaaaat gataaaaccc gtcgggtact ggaaaaagct
     1621 tttggggaag cttgggcgga acggtatatg agccaagtct tatttgatgt tatccaataa
     1681 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg
     1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat
     1861 cagtgataga gattgacatc cctatcagtg atagagatac tgagcac
//