LOCUS       BBa_S03457              1747 bp    DNA     linear       10-May-2024
DEFINITION  --Specify Parts List--
ACCESSION   BBa_S03457
VERSION     BBa_S03457.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     promoter        1..117
                     /label=BBa_J13023
     CDS             124..834
                     /label=mCherry
     misc_feature    475..475
                     /label=C->T (removing PstI site)
     misc_feature    124..837
                     /label=BBa_J06504
     misc_feature    850..853
                     /label=conserved
     RBS             846..857
                     /label=BBa_B0034
     CDS             867..1483
                     /label=tetR
     misc_feature    1484..1517
                     /label=SsrA
     misc_feature    1524..1548
                     /label=Help:Barcodes
     CDS             864..1523
                     /label=BBa_C0040
     stem_loop       1568..1611
                     /label=stem_loop
     misc_feature    1557..1636
                     /label=BBa_B0010
     terminator      1557..1636
                     /label=BBa_B0010
     stem_loop       1652..1671
                     /label=T7 TE
     misc_feature    1678..1678
                     /label=stop
     polyA_site      1672..1685
                     /label=polya
     misc_feature    1645..1685
                     /label=BBa_B0012
     terminator      1645..1685
                     /label=BBa_B0012
     misc_feature    1694..1712
                     /label=TetR 1
     misc_feature    1694..1747
                     /label=BBa_R0040
     promoter        1713..1718
                     /label=-35
     misc_feature    1719..1737
                     /label=TetR 2
     promoter        1736..1741
                     /label=-10
     promoter        1694..1747
                     /label=BBa_R0040
     misc_feature    846..1747
                     /label=BBa_Q04400
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag
      481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataatac
      841 tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg
      901 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      961 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
     1021 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     1081 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     1141 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     1201 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
     1261 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     1321 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     1381 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     1441 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
     1501 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
     1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1621 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1681 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
     1741 tgagcac
//