LOCUS       BBa_S03514               749 bp    DNA     linear       23-May-2024
DEFINITION  HinLVA : TT
ACCESSION   BBa_S03514
VERSION     BBa_S03514.1  
FEATURES             Location/Qualifiers
     CDS             1..570
                     /label=Hin
     primer_bind     1..3
                     /label=Fwd primer J31001
     misc_feature    571..609
                     /label=LVA
     misc_feature    610..612
                     /label=Stop codon
     primer_bind     551..612
                     /label=Rev primer J31001
     CDS             1..612
                     /label=BBa_J31001
     stem_loop       632..675
                     /label=stem_loop
     misc_feature    621..700
                     /label=BBa_B0010
     terminator      621..700
                     /label=BBa_B0010
     stem_loop       716..735
                     /label=T7 TE
     misc_feature    742..742
                     /label=stop
     polyA_site      736..749
                     /label=polya
     misc_feature    709..749
                     /label=BBa_B0012
     terminator      709..749
                     /label=BBa_B0012
     terminator      621..749
                     /label=BBa_B0015
ORIGIN
        1 atggctacta ttgggtatat tcgggtgtca acaattgacc aaaatatcga tttacagcgt
       61 aatgcgctta ccagtgcaaa ttgtgaccgc atttttgagg accgtatcag tggcaagatt
      121 gcaaaccgcc ccggcctgaa acgagcgtta aagtatgtaa ataaaggcga tactcttgtc
      181 gtctggaaat tagacagact gggccgcagc gtgaaaaacc tggtggcgtt aatatcagaa
      241 ttacatgaac gtggagctca cttccattct ttaaccgata gtattgatac cagtagcgcg
      301 atggggcgat tcttttttca tgtaatgtca gcactggccg agatggagcg agaattaatt
      361 gtcgagcgaa cccttgccgg actggctgcc gccagagcgc aaggacgact gggagggcgc
      421 cctcgggcga tcaacagaca tgaacaggaa cagattagtc ggctattaga gaaaggccat
      481 cctcggcagc aactagctat tatttttggt attggcgtat ctaccttata cagatatttt
      541 ccggcaagcc gtataaaaaa acgaatgaat aggcctgctg caaacgacga aaactacgct
      601 ttagtagctt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      661 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      721 accttcgggt gggcctttct gcgtttata
//