LOCUS       BBa_S03524              1447 bp    DNA     linear       15-May-2024
DEFINITION  TetF : HixC-TT-RE
ACCESSION   BBa_S03524
VERSION     BBa_S03524.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) forward
     primer_bind     1..24
                     /label=Fwd primer J31007
     primer_bind     1172..1191
                     /label=Rev primer J31007
     misc_feature    1189..1191
                     /label=stop
     CDS             1..1191
                     /label=BBa_J31007
     misc_feature    1200..1225
                     /label=BBa_J44000
     stem_loop       1245..1288
                     /label=stem_loop
     misc_feature    1234..1313
                     /label=BBa_B0010
     terminator      1234..1313
                     /label=BBa_B0010
     stem_loop       1329..1348
                     /label=T7 TE
     misc_feature    1355..1355
                     /label=stop
     polyA_site      1349..1362
                     /label=polya
     misc_feature    1322..1362
                     /label=BBa_B0012
     terminator      1322..1362
                     /label=BBa_B0012
     misc_feature    1421..1426
                     /label=Former SpeI site
     misc_feature    1421..1421
                     /label=Mutation of SpeI site
     misc_feature    1371..1447
                     /label=RE Sequence
     misc_feature    1399..1399
                     /label=Originally a C
     misc_feature    1447..1447
                     /label=Insertion right before biobrick ends
     misc_feature    1376..1391
                     /label=Proximal Fis Binding Site
     misc_feature    1425..1439
                     /label=Distal Fis Binding Site
     misc_feature    1371..1447
                     /label=BBa_J3101
ORIGIN
        1 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
       61 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
      121 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
      181 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
      241 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac
      301 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      361 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      421 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      481 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      541 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
      601 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
      661 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
      721 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
      781 ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt tcggcgagaa gcaggccatt
      841 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
      901 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
      961 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     1021 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     1081 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     1141 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta atactagagt
     1201 tatcaaaaac catggttttt gataatacta gagccaggca tcaaataaaa cgaaaggctc
     1261 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1321 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttcgggtgtc
     1381 aacaattgac caaaatattg atttacagcg taatgcgctt tctagtgcaa attgtgaccg
     1441 cattttg
//