LOCUS       BBa_S03526              1447 bp    DNA     linear       14-May-2024
DEFINITION  TetB-HixC-TT-RE
ACCESSION   BBa_S03526
VERSION     BBa_S03526.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     misc_feature    1200..1225
                     /label=BBa_J44000
     misc_feature    1..1225
                     /label=BBa_S03525
     stem_loop       1245..1288
                     /label=stem_loop
     misc_feature    1234..1313
                     /label=BBa_B0010
     terminator      1234..1313
                     /label=BBa_B0010
     stem_loop       1329..1348
                     /label=T7 TE
     misc_feature    1355..1355
                     /label=stop
     polyA_site      1349..1362
                     /label=polya
     misc_feature    1322..1362
                     /label=BBa_B0012
     terminator      1322..1362
                     /label=BBa_B0012
     misc_feature    1421..1426
                     /label=Former SpeI site
     misc_feature    1421..1421
                     /label=Mutation of SpeI site
     misc_feature    1371..1447
                     /label=RE Sequence
     misc_feature    1399..1399
                     /label=Originally a C
     misc_feature    1447..1447
                     /label=Insertion right before biobrick ends
     misc_feature    1376..1391
                     /label=Proximal Fis Binding Site
     misc_feature    1425..1439
                     /label=Distal Fis Binding Site
     misc_feature    1371..1447
                     /label=BBa_J3101
     misc_feature    1234..1447
                     /label=BBa_S03516
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagagt
     1201 tatcaaaaac catggttttt gataatacta gagccaggca tcaaataaaa cgaaaggctc
     1261 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1321 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttcgggtgtc
     1381 aacaattgac caaaatattg atttacagcg taatgcgctt tctagtgcaa attgtgaccg
     1441 cattttg
//