LOCUS       BBa_S03527              1479 bp    DNA     linear       15-May-2024
DEFINITION  HixC-TetF-HixC-TT-RE
ACCESSION   BBa_S03527
VERSION     BBa_S03527.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     CDS             33..1223
                     /label=tetA(C) forward
     primer_bind     33..56
                     /label=Fwd primer J31007
     primer_bind     1204..1223
                     /label=Rev primer J31007
     misc_feature    1221..1223
                     /label=stop
     CDS             33..1223
                     /label=BBa_J31007
     misc_feature    1232..1257
                     /label=BBa_J44000
     stem_loop       1277..1320
                     /label=stem_loop
     misc_feature    1266..1345
                     /label=BBa_B0010
     terminator      1266..1345
                     /label=BBa_B0010
     stem_loop       1361..1380
                     /label=T7 TE
     misc_feature    1387..1387
                     /label=stop
     polyA_site      1381..1394
                     /label=polya
     misc_feature    1354..1394
                     /label=BBa_B0012
     terminator      1354..1394
                     /label=BBa_B0012
     misc_feature    1453..1458
                     /label=Former SpeI site
     misc_feature    1453..1453
                     /label=Mutation of SpeI site
     misc_feature    1403..1479
                     /label=RE Sequence
     misc_feature    1431..1431
                     /label=Originally a C
     misc_feature    1479..1479
                     /label=Insertion right before biobrick ends
     misc_feature    1408..1423
                     /label=Proximal Fis Binding Site
     misc_feature    1457..1471
                     /label=Distal Fis Binding Site
     misc_feature    1403..1479
                     /label=BBa_J3101
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agatgaaatc taacaatgcg ctcatcgtca
       61 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
      121 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
      181 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
      241 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
      301 tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
      361 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
      421 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
      481 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
      541 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
      601 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
      661 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
      721 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
      781 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggccccg
      841 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
      901 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
      961 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     1021 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     1081 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     1141 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     1201 ggagccgggc cacctcgacc taatactaga gttatcaaaa accatggttt ttgataatac
     1261 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1321 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1381 tttctgcgtt tatatactag agttcgggtg tcaacaattg accaaaatat tgatttacag
     1441 cgtaatgcgc tttctagtgc aaattgtgac cgcattttg
//