LOCUS       BBa_S03528              1481 bp    DNA     linear       14-May-2024
DEFINITION  HixC-TetB-HixC-TT-RE
ACCESSION   BBa_S03528
VERSION     BBa_S03528.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     CDS             35..1225
                     /label=tetA(C) backwards
     misc_feature    35..37
                     /label=stop
     primer_bind     35..54
                     /label=Rev primer J31006
     primer_bind     1202..1225
                     /label=Fwd primer J31006
     CDS             35..1225
                     /label=BBa_J31006
     misc_feature    1234..1259
                     /label=BBa_J44000
     misc_feature    35..1259
                     /label=BBa_S03525
     stem_loop       1279..1322
                     /label=stem_loop
     misc_feature    1268..1347
                     /label=BBa_B0010
     terminator      1268..1347
                     /label=BBa_B0010
     stem_loop       1363..1382
                     /label=T7 TE
     misc_feature    1389..1389
                     /label=stop
     polyA_site      1383..1396
                     /label=polya
     misc_feature    1356..1396
                     /label=BBa_B0012
     terminator      1356..1396
                     /label=BBa_B0012
     misc_feature    1455..1460
                     /label=Former SpeI site
     misc_feature    1455..1455
                     /label=Mutation of SpeI site
     misc_feature    1405..1481
                     /label=RE Sequence
     misc_feature    1433..1433
                     /label=Originally a C
     misc_feature    1481..1481
                     /label=Insertion right before biobrick ends
     misc_feature    1410..1425
                     /label=Proximal Fis Binding Site
     misc_feature    1459..1473
                     /label=Distal Fis Binding Site
     misc_feature    1405..1481
                     /label=BBa_J3101
     misc_feature    1268..1481
                     /label=BBa_S03516
     misc_feature    35..1481
                     /label=BBa_S03526
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagttaggt cgaggtggcc cggctccatg
       61 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
      121 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
      181 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
      241 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
      301 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
      361 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
      421 ggccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
      481 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
      541 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
      601 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
      661 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
      721 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
      781 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
      841 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
      901 tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga
      961 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
     1021 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
     1081 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
     1141 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     1201 cgatgagcgc attgttagat ttcattacta gagttatcaa aaaccatggt ttttgataat
     1261 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1321 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1381 cctttctgcg tttatatact agagttcggg tgtcaacaat tgaccaaaat attgatttac
     1441 agcgtaatgc gctttctagt gcaaattgtg accgcatttt g
//