LOCUS       BBa_S03534              1470 bp    DNA     linear       19-May-2024
DEFINITION  TetB-RBS<sub>rev</sub>-HixC-TT-RE
ACCESSION   BBa_S03534
VERSION     BBa_S03534.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     RBS             1200..1214
                     /label=Reverse RBS
     RBS             1200..1214
                     /label=BBa_J44001
     misc_feature    1..1214
                     /label=BBa_S03532
     misc_feature    1223..1248
                     /label=BBa_J44000
     stem_loop       1268..1311
                     /label=stem_loop
     misc_feature    1257..1336
                     /label=BBa_B0010
     terminator      1257..1336
                     /label=BBa_B0010
     stem_loop       1352..1371
                     /label=T7 TE
     misc_feature    1378..1378
                     /label=stop
     polyA_site      1372..1385
                     /label=polya
     misc_feature    1345..1385
                     /label=BBa_B0012
     terminator      1345..1385
                     /label=BBa_B0012
     misc_feature    1444..1449
                     /label=Former SpeI site
     misc_feature    1444..1444
                     /label=Mutation of SpeI site
     misc_feature    1394..1470
                     /label=RE Sequence
     misc_feature    1422..1422
                     /label=Originally a C
     misc_feature    1470..1470
                     /label=Insertion right before biobrick ends
     misc_feature    1399..1414
                     /label=Proximal Fis Binding Site
     misc_feature    1448..1462
                     /label=Distal Fis Binding Site
     misc_feature    1394..1470
                     /label=BBa_J3101
     misc_feature    1223..1470
                     /label=BBa_S03522
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagagt
     1201 ttctcctctt taattactag agttatcaaa aaccatggtt tttgataata ctagagccag
     1261 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1321 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1381 ttatatacta gagttcgggt gtcaacaatt gaccaaaata ttgatttaca gcgtaatgcg
     1441 ctttctagtg caaattgtga ccgcattttg
//