LOCUS       BBa_S03537               939 bp    DNA     linear       01-Jun-2024
DEFINITION  pLac-RBS-Hin-TT
ACCESSION   BBa_S03537
VERSION     BBa_S03537.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    299..304
                     /label=Former SpeI site
     misc_feature    301..301
                     /label=Mutation to delete the SpeI site
     CDS             230..802
                     /label=Hin sequence
     primer_bind     230..232
                     /label=Fwd primer J31001
     primer_bind     780..799
                     /label=Rev primer J31001
     CDS             230..802
                     /label=BBa_J31000
     stem_loop       822..865
                     /label=stem_loop
     misc_feature    811..890
                     /label=BBa_B0010
     terminator      811..890
                     /label=BBa_B0010
     stem_loop       906..925
                     /label=T7 TE
     misc_feature    932..932
                     /label=stop
     polyA_site      926..939
                     /label=polya
     misc_feature    899..939
                     /label=BBa_B0012
     terminator      899..939
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat
      241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac
      301 cagtgcaaat tgtgaccgca tttttgaaga ccgtatcagt ggcaagattg caaaccgccc
      361 cggcctgaaa cgggcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt
      421 agacagactg ggccgtagcg tgaaaaatct ggtggcgtta atatcagaat tacatgaacg
      481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt
      541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaatcg tcgagcgaac
      601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat
      661 caacaaacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca
      721 attagctatt atttttggta ttggcgtatc caccttatac agatactttc cggcaagcag
      781 tataaaaaaa cgaatgaatt aatactagag ccaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      901 acactggctc accttcgggt gggcctttct gcgtttata
//