LOCUS       BBa_S03563              1384 bp    DNA     linear       09-May-2024
DEFINITION  pBAD-hixC-RBS-TetF
ACCESSION   BBa_S03563
VERSION     BBa_S03563.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=promoter
     promoter        1..130
                     /label=BBa_J44002
     misc_feature    139..164
                     /label=BBa_J44000
     RBS             173..187
                     /label=RBS-1\Strong
     RBS             180..184
                     /label=RBS
     misc_feature    173..187
                     /label=BBa_B0030
     RBS             173..187
                     /label=BBa_B0030
     CDS             194..1384
                     /label=tetA(C) forward
     primer_bind     194..217
                     /label=Fwd primer J31007
     primer_bind     1365..1384
                     /label=Rev primer J31007
     misc_feature    1382..1384
                     /label=stop
     CDS             194..1384
                     /label=BBa_J31007
ORIGIN
        1 gctagcccaa aaaaacggta tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta
       61 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
      121 taatcaatgt tactagagtt atcaaaaacc atggtttttg ataatactag agattaaaga
      181 ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
      241 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
      301 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca
      361 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct
      421 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
      481 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      541 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      601 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      661 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      721 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
      781 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
      841 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
      901 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
      961 aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac gtttcggcga
     1021 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     1081 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     1141 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     1201 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac
     1261 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     1321 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     1381 ctaa
//