LOCUS       BBa_S03564              2654 bp    DNA     linear       12-May-2024
DEFINITION  hixC-pBAD-hixC-RBS-TetF-TT-RE
ACCESSION   BBa_S03564
VERSION     BBa_S03564.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1212
                     /label=tetA(C) forward
     primer_bind     22..45
                     /label=Fwd primer J31007
     primer_bind     1193..1212
                     /label=Rev primer J31007
     misc_feature    1210..1212
                     /label=stop
     CDS             22..1212
                     /label=BBa_J31007
     misc_feature    1..1212
                     /label=BBa_S03562
     RBS             1221..1235
                     /label=RBS-1\Strong
     RBS             1228..1232
                     /label=RBS
     misc_feature    1221..1235
                     /label=BBa_B0030
     RBS             1221..1235
                     /label=BBa_B0030
     CDS             1242..2432
                     /label=tetA(C) forward
     primer_bind     1242..1265
                     /label=Fwd primer J31007
     primer_bind     2413..2432
                     /label=Rev primer J31007
     misc_feature    2430..2432
                     /label=stop
     CDS             1242..2432
                     /label=BBa_J31007
     stem_loop       2452..2495
                     /label=stem_loop
     misc_feature    2441..2520
                     /label=BBa_B0010
     terminator      2441..2520
                     /label=BBa_B0010
     stem_loop       2536..2555
                     /label=T7 TE
     misc_feature    2562..2562
                     /label=stop
     polyA_site      2556..2569
                     /label=polya
     misc_feature    2529..2569
                     /label=BBa_B0012
     terminator      2529..2569
                     /label=BBa_B0012
     terminator      2441..2569
                     /label=BBa_B0015
     misc_feature    2628..2633
                     /label=Former SpeI site
     misc_feature    2628..2628
                     /label=Mutation of SpeI site
     misc_feature    2578..2654
                     /label=RE Sequence
     misc_feature    2606..2606
                     /label=Originally a C
     misc_feature    2654..2654
                     /label=Insertion right before biobrick ends
     misc_feature    2583..2598
                     /label=Proximal Fis Binding Site
     misc_feature    2632..2646
                     /label=Distal Fis Binding Site
     misc_feature    2578..2654
                     /label=BBa_J3101
ORIGIN
        1 attaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
       61 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
      121 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
      181 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
      241 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
      301 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      361 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      421 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      481 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      541 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
      601 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
      661 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
      721 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
      781 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc caccaaacgt
      841 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
      901 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
      961 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     1021 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     1081 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     1141 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     1201 acctcgacct aatactagag attaaagagg agaaatacta gatgaaatct aacaatgcgc
     1261 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
     1321 tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg
     1381 tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt
     1441 ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact
     1501 acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg
     1561 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     1621 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     1681 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     1741 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     1801 gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     1861 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     1921 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     1981 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     2041 ctggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     2101 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     2161 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     2221 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa
     2281 cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
     2341 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
     2401 gcggtgcatg gagccgggcc acctcgacct aatactagag ccaggcatca aataaaacga
     2461 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2521 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttc
     2581 gggtgtcaac aattgaccaa aatattgatt tacagcgtaa tgcgctttct agtgcaaatt
     2641 gtgaccgcat tttg
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