LOCUS BBa_S03565 2826 bp DNA linear 11-May-2024 DEFINITION hixC-pBADrev-hixC-RBS-TetF-TT-RE ACCESSION BBa_S03565 VERSION BBa_S03565.1 FEATURES Location/Qualifiers promoter 1..130 /label=promoter promoter 1..130 /label=BBa_J44002 misc_feature 139..164 /label=BBa_J44000 RBS 173..187 /label=RBS-1\Strong RBS 180..184 /label=RBS misc_feature 173..187 /label=BBa_B0030 RBS 173..187 /label=BBa_B0030 CDS 194..1384 /label=tetA(C) forward primer_bind 194..217 /label=Fwd primer J31007 primer_bind 1365..1384 /label=Rev primer J31007 misc_feature 1382..1384 /label=stop CDS 194..1384 /label=BBa_J31007 misc_feature 1..1384 /label=BBa_S03563 RBS 1393..1407 /label=RBS-1\Strong RBS 1400..1404 /label=RBS misc_feature 1393..1407 /label=BBa_B0030 RBS 1393..1407 /label=BBa_B0030 CDS 1414..2604 /label=tetA(C) forward primer_bind 1414..1437 /label=Fwd primer J31007 primer_bind 2585..2604 /label=Rev primer J31007 misc_feature 2602..2604 /label=stop CDS 1414..2604 /label=BBa_J31007 stem_loop 2624..2667 /label=stem_loop misc_feature 2613..2692 /label=BBa_B0010 terminator 2613..2692 /label=BBa_B0010 stem_loop 2708..2727 /label=T7 TE misc_feature 2734..2734 /label=stop polyA_site 2728..2741 /label=polya misc_feature 2701..2741 /label=BBa_B0012 terminator 2701..2741 /label=BBa_B0012 terminator 2613..2741 /label=BBa_B0015 misc_feature 2800..2805 /label=Former SpeI site misc_feature 2800..2800 /label=Mutation of SpeI site misc_feature 2750..2826 /label=RE Sequence misc_feature 2778..2778 /label=Originally a C misc_feature 2826..2826 /label=Insertion right before biobrick ends misc_feature 2755..2770 /label=Proximal Fis Binding Site misc_feature 2804..2818 /label=Distal Fis Binding Site misc_feature 2750..2826 /label=BBa_J3101 ORIGIN 1 gctagcccaa aaaaacggta tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta 61 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa 121 taatcaatgt tactagagtt atcaaaaacc atggtttttg ataatactag agattaaaga 181 ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct 241 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt 301 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca 361 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct 421 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct 481 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc 541 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat 601 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc 661 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact 721 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc 781 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 841 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 901 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 961 aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac gtttcggcga 1021 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 1081 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 1141 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 1201 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac 1261 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 1321 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 1381 ctaatactag agattaaaga ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc 1441 atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg 1501 ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta 1561 gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact gtccgaccgc 1621 tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga ctacgcgatc 1681 atggcgacca cacccgtcct gtggatcctc tacgccggac gcatcgtggc cggcatcacc 1741 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 1801 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 1861 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 1921 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 1981 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 2041 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 2101 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 2161 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggcccc 2221 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 2281 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 2341 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 2401 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 2461 attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 2521 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 2581 tggagccggg ccacctcgac ctaatactag agccaggcat caaataaaac gaaaggctca 2641 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2701 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt tcgggtgtca 2761 acaattgacc aaaatattga tttacagcgt aatgcgcttt ctagtgcaaa ttgtgaccgc 2821 attttg //