LOCUS       BBa_S03588              1922 bp    DNA     linear       07-May-2024
DEFINITION  R0079:S03586
ACCESSION   BBa_S03588
VERSION     BBa_S03588.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     misc_feature    184..186
                     /label=start
     misc_feature    967..972
                     /label=2
     misc_feature    934..966
                     /label=LVA
     CDS             184..933
                     /label=aiiA
     misc_feature    184..972
                     /label=BBa_C0060
     misc_feature    973..997
                     /label=Help:Barcodes
     CDS             184..972
                     /label=BBa_C0060
     misc_feature    1010..1013
                     /label=conserved
     RBS             1006..1017
                     /label=BBa_B0034
     misc_feature    1780..1785
                     /label=stop
     misc_feature    1741..1779
                     /label=AAV
     misc_feature    1024..1785
                     /label=BBa_E0034
     stem_loop       1805..1848
                     /label=stem_loop
     misc_feature    1794..1873
                     /label=BBa_B0010
     terminator      1794..1873
                     /label=BBa_B0010
     stem_loop       1889..1908
                     /label=T7 TE
     misc_feature    1915..1915
                     /label=stop
     polyA_site      1909..1922
                     /label=polya
     misc_feature    1882..1922
                     /label=BBa_B0012
     terminator      1882..1922
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatgacag taaagaagct ttatttcgtc ccagcaggtc gttgtatgtt ggatcattcg
      241 tctgttaata gtacattaac accaggagaa ttattagact taccggtttg gtgttatctt
      301 ttggagactg aagaaggacc tattttagta gatacaggta tgccagaaag tgcagttaat
      361 aatgaaggtc tttttaacgg tacatttgtc gaagggcagg ttttaccgaa aatgactgaa
      421 gaagatagaa tcgtgaatat tttaaaacgg gttggttatg agccggaaga ccttctttat
      481 attattagtt ctcacttgca ttttgatcat gcaggaggaa atggcgcttt tataaataca
      541 ccaatcattg tacagcgtgc tgaatatgag gcggcgcagc atagcgaaga atatttgaaa
      601 gaatgtatat tgccgaattt aaactacaaa atcattgaag gtgattatga agtcgtacca
      661 ggagttcaat tattgcatac accaggccat actccagggc atcaatcgct attaattgag
      721 acagaaaaat ccggtcctgt attattaacg attgatgcat cgtatacgaa agagaatttt
      781 gaaaatgaag tgccatttgc gggatttgat tcagaattag ctttatcttc aattaaacgt
      841 ttaaaagaag tggtgatgaa agagaagccg attgttttct ttggacatga tatagagcag
      901 gaaaggggat gtaaagtgtt ccctgaatat atagctgcaa acgacgaaaa ctacgcttta
      961 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagaaaga ggagaaatac
     1021 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1081 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1141 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1201 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
     1261 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1321 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1381 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1441 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1501 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1561 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1621 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1681 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1741 aggcctgctg caaacgacga aaactacgct gctgctgttt aataatacta gagccaggca
     1801 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1861 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1921 ta
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