LOCUS       BBa_S03634              1468 bp    DNA     linear       17-May-2024
DEFINITION  RBS-TetF : HixC-TT-RE
ACCESSION   BBa_S03634
VERSION     BBa_S03634.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1212
                     /label=tetA(C) forward
     primer_bind     22..45
                     /label=Fwd primer J31007
     primer_bind     1193..1212
                     /label=Rev primer J31007
     misc_feature    1210..1212
                     /label=stop
     CDS             22..1212
                     /label=BBa_J31007
     misc_feature    1221..1246
                     /label=BBa_J44000
     stem_loop       1266..1309
                     /label=stem_loop
     misc_feature    1255..1334
                     /label=BBa_B0010
     terminator      1255..1334
                     /label=BBa_B0010
     stem_loop       1350..1369
                     /label=T7 TE
     misc_feature    1376..1376
                     /label=stop
     polyA_site      1370..1383
                     /label=polya
     misc_feature    1343..1383
                     /label=BBa_B0012
     terminator      1343..1383
                     /label=BBa_B0012
     misc_feature    1442..1447
                     /label=Former SpeI site
     misc_feature    1442..1442
                     /label=Mutation of SpeI site
     misc_feature    1392..1468
                     /label=RE Sequence
     misc_feature    1420..1420
                     /label=Originally a C
     misc_feature    1468..1468
                     /label=Insertion right before biobrick ends
     misc_feature    1397..1412
                     /label=Proximal Fis Binding Site
     misc_feature    1446..1460
                     /label=Distal Fis Binding Site
     misc_feature    1392..1468
                     /label=BBa_J3101
ORIGIN
        1 attaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
       61 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
      121 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
      181 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
      241 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
      301 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      361 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      421 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      481 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      541 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
      601 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
      661 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
      721 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
      781 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc caccaaacgt
      841 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
      901 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
      961 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     1021 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     1081 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     1141 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     1201 acctcgacct aatactagag ttatcaaaaa ccatggtttt tgataatact agagccaggc
     1261 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1321 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1381 atatactaga gttcgggtgt caacaattga ccaaaatatt gatttacagc gtaatgcgct
     1441 ttctagtgca aattgtgacc gcattttg
//