LOCUS       BBa_S03635              1674 bp    DNA     linear       20-May-2024
DEFINITION  HixC-pBad-HixC-RBS-TetF : HixC-TT-RE
ACCESSION   BBa_S03635
VERSION     BBa_S03635.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     promoter        35..164
                     /label=BBa_I13453
     misc_feature    173..198
                     /label=BBa_J44000
     RBS             207..221
                     /label=RBS-1\Strong
     RBS             214..218
                     /label=RBS
     misc_feature    207..221
                     /label=BBa_B0030
     RBS             207..221
                     /label=BBa_B0030
     CDS             228..1418
                     /label=tetA(C) forward
     primer_bind     228..251
                     /label=Fwd primer J31007
     primer_bind     1399..1418
                     /label=Rev primer J31007
     misc_feature    1416..1418
                     /label=stop
     CDS             228..1418
                     /label=BBa_J31007
     misc_feature    1427..1452
                     /label=BBa_J44000
     stem_loop       1472..1515
                     /label=stem_loop
     misc_feature    1461..1540
                     /label=BBa_B0010
     terminator      1461..1540
                     /label=BBa_B0010
     stem_loop       1556..1575
                     /label=T7 TE
     misc_feature    1582..1582
                     /label=stop
     polyA_site      1576..1589
                     /label=polya
     misc_feature    1549..1589
                     /label=BBa_B0012
     terminator      1549..1589
                     /label=BBa_B0012
     misc_feature    1648..1653
                     /label=Former SpeI site
     misc_feature    1648..1648
                     /label=Mutation of SpeI site
     misc_feature    1598..1674
                     /label=RE Sequence
     misc_feature    1626..1626
                     /label=Originally a C
     misc_feature    1674..1674
                     /label=Insertion right before biobrick ends
     misc_feature    1603..1618
                     /label=Proximal Fis Binding Site
     misc_feature    1652..1666
                     /label=Distal Fis Binding Site
     misc_feature    1598..1674
                     /label=BBa_J3101
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagacattg attatttgca cggcgtcaca
       61 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
      121 tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag agttatcaaa
      181 aaccatggtt tttgataata ctagagatta aagaggagaa atactagatg aaatctaaca
      241 atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta
      301 tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact
      361 atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag
      421 cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta
      481 tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc ggacgcatcg
      541 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
      601 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
      661 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
      721 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
      781 ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
      841 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
      901 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
      961 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     1021 tcgtcactgg ccccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     1081 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     1141 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     1201 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     1261 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
     1321 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
     1381 tgcgtcgcgg tgcatggagc cgggccacct cgacctaata ctagagttat caaaaaccat
     1441 ggtttttgat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1501 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1561 accttcgggt gggcctttct gcgtttatat actagagttc gggtgtcaac aattgaccaa
     1621 aatattgatt tacagcgtaa tgcgctttct agtgcaaatt gtgaccgcat tttg
//