LOCUS       BBa_S03642              1454 bp    DNA     linear       13-May-2024
DEFINITION  (-2,-1) HixC-TetB-RBS<sub>rev</sub>-HixC : pBad<sub>rev</sub>-HixC
ACCESSION   BBa_S03642
VERSION     BBa_S03642.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     CDS             35..1225
                     /label=tetA(C) backwards
     misc_feature    35..37
                     /label=stop
     primer_bind     35..54
                     /label=Rev primer J31006
     primer_bind     1202..1225
                     /label=Fwd primer J31006
     CDS             35..1225
                     /label=BBa_J31006
     RBS             1234..1248
                     /label=Reverse RBS
     RBS             1234..1248
                     /label=BBa_J44001
     misc_feature    1257..1282
                     /label=BBa_J44000
     promoter        1291..1420
                     /label=promoter
     promoter        1291..1420
                     /label=BBa_J44002
     misc_feature    1429..1454
                     /label=BBa_J44000
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagttaggt cgaggtggcc cggctccatg
       61 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
      121 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
      181 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
      241 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
      301 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
      361 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
      421 ggccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
      481 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
      541 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
      601 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
      661 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
      721 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
      781 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
      841 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
      901 tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga
      961 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
     1021 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
     1081 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
     1141 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     1201 cgatgagcgc attgttagat ttcattacta gagtttctcc tctttaatta ctagagttat
     1261 caaaaaccat ggtttttgat aatactagag gctagcccaa aaaaacggta tggagaaaca
     1321 gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga taaaaatgct
     1381 atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt tactagagtt atcaaaaacc
     1441 atggtttttg ataa
//