LOCUS       BBa_S03647              1454 bp    DNA     linear       19-May-2024
DEFINITION  pLac-HixC : RBS-TetF
ACCESSION   BBa_S03647
VERSION     BBa_S03647.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..234
                     /label=BBa_J44000
     RBS             243..257
                     /label=RBS-1\Strong
     RBS             250..254
                     /label=RBS
     misc_feature    243..257
                     /label=BBa_B0030
     RBS             243..257
                     /label=BBa_B0030
     CDS             264..1454
                     /label=tetA(C) forward
     primer_bind     264..287
                     /label=Fwd primer J31007
     primer_bind     1435..1454
                     /label=Rev primer J31007
     misc_feature    1452..1454
                     /label=stop
     CDS             264..1454
                     /label=BBa_J31007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtt atcaaaaacc atggtttttg ataatactag
      241 agattaaaga ggagaaatac tagatgaaat ctaacaatgc gctcatcgtc atcctcggca
      301 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc
      361 gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg
      421 cgttgatgca atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc
      481 gcccagtcct gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca
      541 cacccgtcct gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      601 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      661 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      721 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
      781 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc
      841 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
      901 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
      961 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     1021 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggcccc gccaccaaac
     1081 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     1141 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     1201 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     1261 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc
     1321 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     1381 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     1441 ccacctcgac ctaa
//