LOCUS       BBa_S03653               925 bp    DNA     linear       24-Apr-2024
DEFINITION  E0042:S03650
ACCESSION   BBa_S03653
VERSION     BBa_S03653.1  
FEATURES             Location/Qualifiers
     misc_feature    1..7
                     /label=SapI recognition
     misc_feature    1..8
                     /label=BBa_B0102
     CDS             9..719
                     /label=mCherry
     misc_feature    360..360
                     /label=C->T (removing PstI site)
     misc_feature    9..722
                     /label=BBa_J06504
     misc_feature    1..722
                     /label=BBa_E0042
     stem_loop       736..788
                     /label=stem_loop
     misc_feature    731..788
                     /label=BBa_B0101
     stem_loop       808..851
                     /label=stem_loop
     misc_feature    797..876
                     /label=BBa_B0010
     terminator      797..876
                     /label=BBa_B0010
     stem_loop       892..911
                     /label=T7 TE
     misc_feature    918..918
                     /label=stop
     polyA_site      912..925
                     /label=polya
     misc_feature    885..925
                     /label=BBa_B0012
     terminator      885..925
                     /label=BBa_B0012
ORIGIN
        1 gctcttctat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
       61 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      121 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      181 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      241 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      301 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct
      361 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      421 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      481 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      541 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      601 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      661 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaat
      721 aatactagag gatcgcctga tcccggtgca cccgggcagc tgcatagtct gggtgcaccg
      781 ggatcaggta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      841 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      901 tcgggtgggc ctttctgcgt ttata
//