LOCUS       BBa_S03679              2231 bp    DNA     linear       14-May-2024
DEFINITION  (2,-1) RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-RBS-TetF-HixC : 
            pLac<sub>rev</sub>-HixC
ACCESSION   BBa_S03679
VERSION     BBa_S03679.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..735
                     /label=BBa_J44000
     RBS             744..758
                     /label=RBS-1\Strong
     RBS             751..755
                     /label=RBS
     misc_feature    744..758
                     /label=BBa_B0030
     RBS             744..758
                     /label=BBa_B0030
     CDS             765..1955
                     /label=tetA(C) forward
     primer_bind     765..788
                     /label=Fwd primer J31007
     primer_bind     1936..1955
                     /label=Rev primer J31007
     misc_feature    1953..1955
                     /label=stop
     CDS             765..1955
                     /label=BBa_J31007
     misc_feature    1964..1989
                     /label=BBa_J44000
     misc_feature    1998..2197
                     /label=R0010 reverse
     CDS             2110..2197
                     /label=end of LacI coding region (inactive)
     misc_feature    2071..2109
                     /label=CAP binding site
     promoter        2055..2061
                     /label=-35
     promoter        2032..2037
                     /label=-10
     misc_feature    1998..2031
                     /label=LacI binding site
     promoter        1998..2197
                     /label=BBa_J31013
     misc_feature    2206..2231
                     /label=BBa_J44000
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
      721 catggttttt gataatacta gagattaaag aggagaaata ctagatgaaa tctaacaatg
      781 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
      841 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
      901 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
      961 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1021 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1081 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1141 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1201 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1261 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1321 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1381 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     1441 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1501 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1561 tcactggccc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1621 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     1681 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     1741 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     1801 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     1861 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     1921 gtcgcggtgc atggagccgg gccacctcga cctaatacta gagttatcaa aaaccatggt
     1981 ttttgataat actagagtgt gtgaaattgt tatccgctca caattccaca caacatacga
     2041 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2101 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2161 atcggccaac gcgcggggag aggcggtttg cgtattgtac tagagttatc aaaaaccatg
     2221 gtttttgata a
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