LOCUS       BBa_S03681              2233 bp    DNA     linear       15-May-2024
DEFINITION  (-2,-1) RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-TB-RBS<sub>rev</su
            b>-HixC : pLac<sub>rev</sub>-HixC
ACCESSION   BBa_S03681
VERSION     BBa_S03681.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..735
                     /label=BBa_J44000
     CDS             744..1934
                     /label=tetA(C) backwards
     misc_feature    744..746
                     /label=stop
     primer_bind     744..763
                     /label=Rev primer J31006
     primer_bind     1911..1934
                     /label=Fwd primer J31006
     CDS             744..1934
                     /label=BBa_J31006
     RBS             1943..1957
                     /label=Reverse RBS
     RBS             1943..1957
                     /label=BBa_J44001
     misc_feature    1966..1991
                     /label=BBa_J44000
     misc_feature    2000..2199
                     /label=R0010 reverse
     CDS             2112..2199
                     /label=end of LacI coding region (inactive)
     misc_feature    2073..2111
                     /label=CAP binding site
     promoter        2057..2063
                     /label=-35
     promoter        2034..2039
                     /label=-10
     misc_feature    2000..2033
                     /label=LacI binding site
     promoter        2000..2199
                     /label=BBa_J31013
     misc_feature    2208..2233
                     /label=BBa_J44000
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
      721 catggttttt gataatacta gagttaggtc gaggtggccc ggctccatgc accgcgacgc
      781 aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat
      841 gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat cgaagttagg
      901 ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
      961 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     1021 gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc
     1081 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg gccagtgacg
     1141 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
     1201 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
     1261 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
     1321 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta
     1381 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     1441 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     1501 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     1561 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     1621 acgatgcgtc cggcgtagag gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
     1681 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
     1741 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
     1801 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
     1861 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
     1921 ttgttagatt tcattactag agtttctcct ctttaattac tagagttatc aaaaaccatg
     1981 gtttttgata atactagagt gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2041 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2101 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2161 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgt actagagtta tcaaaaacca
     2221 tggtttttga taa
//