LOCUS       BBa_S03686               977 bp    DNA     linear       14-May-2024
DEFINITION  RFP<sub>rev</sub>RBS<sub>rev</sub>-HixC : pLac<sub>rev</sub>-HixC 
            (dummy part)
ACCESSION   BBa_S03686
VERSION     BBa_S03686.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..735
                     /label=BBa_J44000
     misc_feature    744..943
                     /label=R0010 reverse
     CDS             856..943
                     /label=end of LacI coding region (inactive)
     misc_feature    817..855
                     /label=CAP binding site
     promoter        801..807
                     /label=-35
     promoter        778..783
                     /label=-10
     misc_feature    744..777
                     /label=LacI binding site
     promoter        744..943
                     /label=BBa_J31013
     misc_feature    952..977
                     /label=BBa_J44000
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagagt tatcaaaaac
      721 catggttttt gataatacta gagtgtgtga aattgttatc cgctcacaat tccacacaac
      781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgtactaga gttatcaaaa
      961 accatggttt ttgataa
//