BBa_J31013
1
BBa_J31013
pLac Backwards [cf. BBa_R0010]
2007-04-11T11:00:00Z
2015-08-31T04:08:45Z
BBa_R0010
Promoter from the lack operon cloned in the reverse orientation.
false
false
_61_
0
1144
61
Not in stock
false
Promoter from the lack operon cloned in the reverse orientation.
false
Karmella Haynes
annotation1928673
1
LacI binding site
range1928673
1
1
34
annotation1928670
1
CAP binding site
range1928670
1
74
112
annotation1928671
1
-35
range1928671
1
58
64
annotation1928672
1
-10
range1928672
1
35
40
annotation1928669
1
end of LacI coding region (inactive)
range1928669
1
113
200
annotation1928668
1
R0010 reverse
range1928668
1
1
200
BBa_S03712
1
BBa_S03712
pLacB-RBS.1
2007-06-18T11:00:00Z
2015-05-08T01:14:29Z
false
false
_120_
0
1491
9
It's complicated
false
false
Will DeLoache
component1934954
1
BBa_B0030
component1934952
1
BBa_J31013
annotation1934952
1
BBa_J31013
range1934952
1
1
200
annotation1934954
1
BBa_B0030
range1934954
1
209
223
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_S03712_sequence
1
tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgtactagagattaaagaggagaaa
BBa_J31013_sequence
1
tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z