LOCUS       BBa_S03715              1251 bp    DNA     linear       10-May-2024
DEFINITION  lox-RBS-Tet
ACCESSION   BBa_S03715
VERSION     BBa_S03715.1  
FEATURES             Location/Qualifiers
     misc_feature    1..34
                     /label=BBa_J61046
     misc_feature    47..50
                     /label=conserved
     RBS             43..54
                     /label=BBa_B0034
     CDS             61..1251
                     /label=tetA(C) forward
     primer_bind     61..84
                     /label=Fwd primer J31007
     primer_bind     1232..1251
                     /label=Rev primer J31007
     misc_feature    1249..1251
                     /label=stop
     CDS             61..1251
                     /label=BBa_J31007
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttattactag agaaagagga gaaatactag
       61 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
      121 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
      181 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
      241 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
      301 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac
      361 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      421 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      481 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      541 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      601 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
      661 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
      721 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
      781 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
      841 ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt tcggcgagaa gcaggccatt
      901 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
      961 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     1021 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     1081 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     1141 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     1201 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta a
//