LOCUS       BBa_S03720              1941 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS-Tet-RBS-RFP
ACCESSION   BBa_S03720
VERSION     BBa_S03720.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1209
                     /label=tetA(C) forward
     primer_bind     19..42
                     /label=Fwd primer J31007
     primer_bind     1190..1209
                     /label=Rev primer J31007
     misc_feature    1207..1209
                     /label=stop
     CDS             19..1209
                     /label=BBa_J31007
     misc_feature    1..1209
                     /label=BBa_S03710
     misc_feature    1222..1225
                     /label=conserved
     RBS             1218..1229
                     /label=BBa_B0034
     CDS             1236..1910
                     /label=mrfp1
     misc_feature    1917..1941
                     /label=Help:Barcodes
     CDS             1236..1916
                     /label=BBa_E1010
     misc_feature    1218..1941
                     /label=BBa_I13502
ORIGIN
        1 aaagaggaga aatactagat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
       61 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
      121 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
      181 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
      241 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
      301 gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
      361 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
      421 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
      481 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      541 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg
      601 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
      661 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
      721 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
      781 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gccccgccac caaacgtttc
      841 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
      901 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
      961 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     1021 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc
     1081 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     1141 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     1201 tcgacctaat actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
     1261 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     1321 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     1381 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     1441 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     1501 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     1561 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     1621 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     1681 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     1741 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1801 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1861 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     1921 gatagtgcta gtgtagatcg c
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