LOCUS       BBa_S03721              2783 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS-Kan-RBS-RFP-RBS-Tet
ACCESSION   BBa_S03721
VERSION     BBa_S03721.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..834
                     /label=KanR Gene
     CDS             19..834
                     /label=BBa_J31003
     misc_feature    1..834
                     /label=BBa_S03709
     misc_feature    847..850
                     /label=conserved
     RBS             843..854
                     /label=BBa_B0034
     CDS             861..1535
                     /label=mrfp1
     misc_feature    1542..1566
                     /label=Help:Barcodes
     CDS             861..1541
                     /label=BBa_E1010
     misc_feature    843..1566
                     /label=BBa_I13502
     misc_feature    1..1566
                     /label=BBa_S03711
     misc_feature    1579..1582
                     /label=conserved
     RBS             1575..1586
                     /label=BBa_B0034
     CDS             1593..2783
                     /label=tetA(C) forward
     primer_bind     1593..1616
                     /label=Fwd primer J31007
     primer_bind     2764..2783
                     /label=Rev primer J31007
     misc_feature    2781..2783
                     /label=stop
     CDS             1593..2783
                     /label=BBa_J31007
     misc_feature    1575..2783
                     /label=BBa_S03710
ORIGIN
        1 aaagaggaga aatactagat gagccatatt caacgggaaa cgtcttgctc caggccgcga
       61 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      121 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
      181 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      241 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      301 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct
      361 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
      421 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
      481 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
      541 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
      601 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
      661 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
      721 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
      781 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatactag
      841 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      901 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      961 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
     1021 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
     1081 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
     1141 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
     1201 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     1261 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     1321 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     1381 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     1441 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     1501 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
     1561 gatcgctact agagaaagag gagaaatact agatgaaatc taacaatgcg ctcatcgtca
     1621 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     1681 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     1741 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     1801 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     1861 tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
     1921 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     1981 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     2041 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     2101 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     2161 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     2221 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     2281 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     2341 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggccccg
     2401 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     2461 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     2521 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     2581 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     2641 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     2701 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2761 ggagccgggc cacctcgacc taa
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