LOCUS       BBa_S03729              2074 bp    DNA     linear       11-May-2024
DEFINITION  RFP(reverse)-TT-lox-RBS-Tet
ACCESSION   BBa_S03729
VERSION     BBa_S03729.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     stem_loop       698..741
                     /label=stem_loop
     misc_feature    687..766
                     /label=BBa_B0010
     terminator      687..766
                     /label=BBa_B0010
     stem_loop       782..801
                     /label=T7 TE
     misc_feature    808..808
                     /label=stop
     polyA_site      802..815
                     /label=polya
     misc_feature    775..815
                     /label=BBa_B0012
     terminator      775..815
                     /label=BBa_B0012
     misc_feature    824..857
                     /label=BBa_J61046
     misc_feature    870..873
                     /label=conserved
     RBS             866..877
                     /label=BBa_B0034
     CDS             884..2074
                     /label=tetA(C) forward
     primer_bind     884..907
                     /label=Fwd primer J31007
     primer_bind     2055..2074
                     /label=Rev primer J31007
     misc_feature    2072..2074
                     /label=stop
     CDS             884..2074
                     /label=BBa_J31007
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      721 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      781 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagataactt cgtataatgt
      841 atgctatacg aagttattac tagagaaaga ggagaaatac tagatgaaat ctaacaatgc
      901 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
      961 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
     1021 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     1081 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     1141 ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac gcatcgtggc
     1201 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1261 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1321 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     1381 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1441 gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1501 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1561 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     1621 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     1681 cactggcccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     1741 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     1801 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     1861 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     1921 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     1981 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     2041 tcgcggtgca tggagccggg ccacctcgac ctaa
//