LOCUS BBa_S03737 2324 bp DNA linear 10-May-2024 DEFINITION pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2) ACCESSION BBa_S03737 VERSION BBa_S03737.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..242 /label=BBa_J61046 CDS 251..928 /label=mRFP reverse primer_bind 909..928 /label=Spe mRFP F primer_bind 251..270 /label=Xba mRFP R misc_feature 251..928 /label=BBa_J31008 stem_loop 948..991 /label=stem_loop misc_feature 937..1016 /label=BBa_B0010 terminator 937..1016 /label=BBa_B0010 stem_loop 1032..1051 /label=T7 TE misc_feature 1058..1058 /label=stop polyA_site 1052..1065 /label=polya misc_feature 1025..1065 /label=BBa_B0012 terminator 1025..1065 /label=BBa_B0012 misc_feature 1074..1107 /label=BBa_J61046 misc_feature 1120..1123 /label=conserved RBS 1116..1127 /label=BBa_B0034 CDS 1134..2324 /label=tetA(C) forward primer_bind 1134..1157 /label=Fwd primer J31007 primer_bind 2305..2324 /label=Rev primer J31007 misc_feature 2322..2324 /label=stop CDS 1134..2324 /label=BBa_J31007 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat aacttcgtat aatgtatgct atacgaagtt 241 attactagag ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga 301 tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg 361 gcagctgaac cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac 421 cgtctttcag tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca 481 tacgttcggt ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg 541 ggaagttggt accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt 601 cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac 661 cttccgggaa ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt 721 tggaaccgta ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt 781 tggtaacttt cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt 841 cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact 901 ctttgataac gtcttcggag gaagccatta ctagagccag gcatcaaata aaacgaaagg 961 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1021 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagataactt 1081 cgtataatgt atgctatacg aagttattac tagagaaaga ggagaaatac tagatgaaat 1141 ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct 1201 tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca 1261 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc 1321 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag 1381 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac 1441 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 1501 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 1561 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat 1621 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg 1681 agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 1741 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 1801 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 1861 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 1921 aagccttcgt cactggcccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 1981 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg 2041 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca 2101 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc 2161 ttaccagcct aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg 2221 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc 2281 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctaa // LOCUS BBa_I715013 2324 bp DNA linear 10-May-2024 DEFINITION pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB3K3) ACCESSION BBa_I715013 VERSION BBa_I715013.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..242 /label=BBa_J61046 CDS 251..928 /label=mRFP reverse primer_bind 909..928 /label=Spe mRFP F primer_bind 251..270 /label=Xba mRFP R misc_feature 251..928 /label=BBa_J31008 stem_loop 948..991 /label=stem_loop misc_feature 937..1016 /label=BBa_B0010 terminator 937..1016 /label=BBa_B0010 stem_loop 1032..1051 /label=T7 TE misc_feature 1058..1058 /label=stop polyA_site 1052..1065 /label=polya misc_feature 1025..1065 /label=BBa_B0012 terminator 1025..1065 /label=BBa_B0012 misc_feature 1074..1107 /label=BBa_J61046 misc_feature 1120..1123 /label=conserved RBS 1116..1127 /label=BBa_B0034 CDS 1134..2324 /label=tetA(C) forward primer_bind 1134..1157 /label=Fwd primer J31007 primer_bind 2305..2324 /label=Rev primer J31007 misc_feature 2322..2324 /label=stop CDS 1134..2324 /label=BBa_J31007 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat aacttcgtat aatgtatgct atacgaagtt 241 attactagag ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga 301 tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg 361 gcagctgaac cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac 421 cgtctttcag tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca 481 tacgttcggt ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg 541 ggaagttggt accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt 601 cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac 661 cttccgggaa ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt 721 tggaaccgta ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt 781 tggtaacttt cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt 841 cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact 901 ctttgataac gtcttcggag gaagccatta ctagagccag gcatcaaata aaacgaaagg 961 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1021 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagataactt 1081 cgtataatgt atgctatacg aagttattac tagagaaaga ggagaaatac tagatgaaat 1141 ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct 1201 tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca 1261 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc 1321 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag 1381 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac 1441 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 1501 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 1561 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat 1621 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg 1681 agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 1741 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 1801 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 1861 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 1921 aagccttcgt cactggcccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 1981 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg 2041 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca 2101 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc 2161 ttaccagcct aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg 2221 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc 2281 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctaa //