LOCUS       BBa_S03749              1340 bp    DNA     linear       24-Apr-2024
DEFINITION  tet-regulated cro (Strong tetR promoter)
ACCESSION   BBa_S03749
VERSION     BBa_S03749.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    112..312
                     /label=cro gene
     misc_feature    55..111
                     /label=spacer
     misc_feature    1..312
                     /label=BBa_I759002
     stem_loop       332..375
                     /label=stem_loop
     misc_feature    321..400
                     /label=BBa_B0010
     terminator      321..400
                     /label=BBa_B0010
     stem_loop       416..435
                     /label=T7 TE
     misc_feature    442..442
                     /label=stop
     polyA_site      436..449
                     /label=polya
     misc_feature    409..449
                     /label=BBa_B0012
     terminator      409..449
                     /label=BBa_B0012
     terminator      321..449
                     /label=BBa_B0015
     promoter        458..492
                     /label=BBa_J23100
     misc_feature    505..508
                     /label=conserved
     RBS             501..512
                     /label=BBa_B0034
     CDS             522..1138
                     /label=tetR
     misc_feature    1139..1172
                     /label=SsrA
     misc_feature    1179..1203
                     /label=Help:Barcodes
     CDS             519..1178
                     /label=BBa_C0040
     stem_loop       1223..1266
                     /label=stem_loop
     misc_feature    1212..1291
                     /label=BBa_B0010
     terminator      1212..1291
                     /label=BBa_B0010
     stem_loop       1307..1326
                     /label=T7 TE
     misc_feature    1333..1333
                     /label=stop
     polyA_site      1327..1340
                     /label=polya
     misc_feature    1300..1340
                     /label=BBa_B0012
     terminator      1300..1340
                     /label=BBa_B0012
     misc_feature    501..1340
                     /label=BBa_P0440
     misc_feature    458..1340
                     /label=BBa_S03706
     misc_feature    321..1340
                     /label=BBa_S03745
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 aattcactga gtcatatgat cgatttgggt attaaagagg agaaacaatt gatggaacaa
      121 cgcataaccc tgaaagatta tgcaatgcgc tttgggcaaa ccaagacagc taaagatctc
      181 ggcgtatatc aaagcgcgat caacaaggcc attcatgcag gccgaaagat ttttttaact
      241 ataaacgctg atggaagcgt ttatgcggaa gaggtaaagc ccttcccgag taacaaaaaa
      301 acaacagcat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      361 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      421 accttcgggt gggcctttct gcgtttatat actagagttg acggctagct cagtcctagg
      481 tacagtgcta gctactagag aaagaggaga aatactagat gtccagatta gataaaagta
      541 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
      601 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
      661 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
      721 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
      781 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
      841 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
      901 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
      961 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     1021 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     1081 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg
     1141 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat
     1201 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1261 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1321 tgggcctttc tgcgtttata
//