LOCUS BBa_S03751 5216 bp DNA linear 20-May-2024 DEFINITION I13504:M30109 ACCESSION BBa_S03751 VERSION BBa_S03751.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..738 /label=GFP protein CDS 19..738 /label=BBa_E0040 stem_loop 758..801 /label=stem_loop misc_feature 747..826 /label=BBa_B0010 terminator 747..826 /label=BBa_B0010 stem_loop 842..861 /label=T7 TE misc_feature 868..868 /label=stop polyA_site 862..875 /label=polya misc_feature 835..875 /label=BBa_B0012 terminator 835..875 /label=BBa_B0012 misc_feature 1..875 /label=BBa_I13504 promoter 884..1013 /label=BBa_I13453 misc_feature 1026..1029 /label=conserved RBS 1022..1033 /label=BBa_B0034 misc_feature 1766..1790 /label=Help:Barcodes CDS 1040..1765 /label=BBa_I15008 misc_feature 1803..1806 /label=conserved RBS 1799..1810 /label=BBa_B0034 CDS 1817..2566 /label=PcyA misc_feature 2567..2591 /label=Help:Barcodes CDS 1817..2566 /label=BBa_I15009 stem_loop 2611..2654 /label=stem_loop misc_feature 2600..2679 /label=BBa_B0010 terminator 2600..2679 /label=BBa_B0010 stem_loop 2695..2714 /label=T7 TE misc_feature 2721..2721 /label=stop polyA_site 2715..2728 /label=polya misc_feature 2688..2728 /label=BBa_B0012 terminator 2688..2728 /label=BBa_B0012 misc_feature 2737..2755 /label=TetR 1 misc_feature 2737..2790 /label=BBa_R0040 promoter 2756..2761 /label=-35 misc_feature 2762..2780 /label=TetR 2 promoter 2779..2784 /label=-10 promoter 2737..2790 /label=BBa_R0040 misc_feature 2803..2806 /label=conserved RBS 2799..2810 /label=BBa_B0034 CDS 2817..5054 /label=Cph8 misc_feature 5055..5079 /label=Help:Barcodes CDS 2817..5054 /label=BBa_I15010 stem_loop 5099..5142 /label=stem_loop misc_feature 5088..5167 /label=BBa_B0010 terminator 5088..5167 /label=BBa_B0010 stem_loop 5183..5202 /label=T7 TE misc_feature 5209..5209 /label=stop polyA_site 5203..5216 /label=polya misc_feature 5176..5216 /label=BBa_B0012 terminator 5176..5216 /label=BBa_B0012 misc_feature 884..5216 /label=BBa_M30109 ORIGIN 1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac 901 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga 961 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga 1021 gaaagaggag aaatactaga tgagtgtcaa cttagcttcc cagttgcggg aagggacgaa 1081 aaaatcccac tccatggcgg agaacgtcgg ctttgtcaaa tgcttcctca agggcgttgt 1141 cgagaaaaat tcctaccgta agctggttgg caatctctac tttgtctaca gtgccatgga 1201 agaggaaatg gcaaaattta aggaccatcc catcctcagc cacatttact tccccgaact 1261 caaccgcaaa caaagcctag agcaagacct gcaattctat tacggctcca actggcggca 1321 agaagtgaaa atttctgccg ctggccaagc ctatgtggac cgagtccggc aagtggccgc 1381 tacggcccct gaattgttgg tggcccattc ctacacccgt tacctggggg atctttccgg 1441 cggtcaaatt ctcaagaaaa ttgcccaaaa tgccatgaat ctccacgatg gtggcacagc 1501 tttctatgaa tttgccgaca ttgatgacga aaaggctttt aaaaatacct accgtcaagc 1561 tatgaatgat ctgcccattg accaagccac cgccgaacgg attgtggatg aagccaatga 1621 cgcctttgcc atgaacatga aaatgttcaa cgaacttgaa ggcaacctga tcaaggcgat 1681 cggcattatg gtgttcaaca gcctcacccg tcgccgcagt caaggcagca ccgaagttgg 1741 cctcgccacc tccgaaggct aataacgctg atagtgctag tgtagatcgc tactagagaa 1801 agaggagaaa tactagatgg ccgtcactga tttaagtttg accaattctt ccctgatgcc 1861 tacgttgaac ccgatgattc aacagttggc cctggcgatc gccgctagtt ggcaaagttt 1921 acccctcaag ccctatcaat tgccggagga tttgggctac gtagaaggcc gcctggaagg 1981 ggaaaagtta gtgattgaaa atcggtgcta ccaaacgccc cagtttcgca aaatgcattt 2041 ggagttggcc aaggtgggca aagggttgga tattctccac tgtgtaatgt ttcctgagcc 2101 tttatacggt ctacctttgt ttggctgtga cattgtggcc ggccccggtg gagtaagtgc 2161 ggctattgcg gatctatccc ccacccaaag cgatcgccaa ttgcccgcag cgtaccaaaa 2221 atcattggca gagctaggcc agccagaatt tgagcaacaa cgggaattgc ccccctgggg 2281 agaaatattt tctgaatatt gtttattcat ccgtcccagc aatgtcactg aagaagaaag 2341 atttgtacaa agggtagtgg actttttgca aattcattgt caccaatcca tcgttgccga 2401 acccttgtct gaagctcaaa ctttggagca ccgtcagggg caaattcatt actgccaaca 2461 acaacagaaa aatgataaaa cccgtcgggt actggaaaaa gcttttgggg aagcttgggc 2521 ggaacggtat atgagccaag tcttatttga tgttatccaa taataactct gatagtgcta 2581 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2641 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2701 ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac 2761 atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg 2821 ccaccaccgt acaactcagc gaccaatccc tccgtcagct agaaaccctc gccatccaca 2881 ccgcccacct gattcagccc cacggtttag tggtggtcct gcaagaacca gacctcacca 2941 tcagccaaat tagcgccaac tgcaccggca ttttagggcg atcgccagag gatttgttgg 3001 gcagaaccct aggggaagtg tttgatagct ttcagattga tcccatccag agtcgcctaa 3061 cggccggaca aatcagcagc ctcaacccca gtaaactttg ggcgcgggtc atgggggacg 3121 actttgtcat ttttgacggg gtttttcatc gcaacagtga cggtttattg gtatgtgaac 3181 tcgagccagc ctacacttcc gataatctgc ccttcctcgg tttttatcac atggccaacg 3241 ctgccctgaa tcggttgcgc caacaagcta atctacggga tttctacgat gttattgtcg 3301 aagaagtccg ccgtatgact ggctttgacc gggtgatgct ataccgcttt gatgaaaata 3361 accacggtga tgtcattgcc gaagataaac gggatgatat ggaaccctat ttgggcctgc 3421 actatcccga atcggatatt ccccaacccg cccgtcggct atttatccac aaccccattc 3481 gagtaattcc cgatgtttat ggtgtggcgg tgcccctgac cccagcggtt aaccccagca 3541 ccaaccgagc ggtggattta acagaatcca ttctgcgcag tgcgtaccat tgccacttga 3601 cctatctgaa aaatatgggg gtaggagcgt ctttaaccat ttccctaatt aaggacggcc 3661 atctctgggg gctcattgcc tgccaccatc aaacccccaa agtaattccc tttgaactgc 3721 gtaaagcctg cgaatttttt ggtcgggtgg tgtttagcaa catttccgcc caggaagata 3781 cggaaacctt cgattaccgg gtgcagctgg cggagcatga agcggtttta ttggacaaaa 3841 tgaccacggc ggcggatttt gtcgaaggat taactaatca tcccgatcgc ctgttgggat 3901 taacgggctc ccagggggcg gccatttgct ttggggaaaa attgatttta gtaggggaaa 3961 ccccggacga gaaagcagtg caatatttac tgcaatggtt ggagaatcgg gaagtgcaag 4021 acgttttctt cacctcttcc ctctcacaaa tttatcctga tgcagtgaat tttaaatccg 4081 tggccagtgg cttattggcc attcccattg cccgtcacaa ctttttgctc tggtttcgcc 4141 ctgaagtgtt gcaaacggtt aattggggcg gtgacccaaa tcatgcttac gaagctaccc 4201 aggaagacgg taaaatcgag ctccatcccc gccaatcctt tgacctctgg aaagaaattg 4261 tccgactcca atctttgccc tggcaatcgg tggaaatcca aagtgccctg gccctgaaaa 4321 aggcgatcgt caacctcatt ttgcgccagg cagaagaatt gcatatggcg gctggtgtta 4381 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc 4441 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag 4501 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc 4561 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagta ctcggtgagg 4621 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca 4681 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg 4741 ccgcccgtta tggcaatggc tgggtcaaag tcagcagcgg aacggagccg aatcgcgcct 4801 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc 4861 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa 4921 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg 4981 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcatgacaa 5041 aagaagggta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat 5101 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 5161 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //