LOCUS       BBa_S03785              1613 bp    DNA     linear       03-Jun-2024
DEFINITION  hixC-RFP2-RBS-GFP1-hixC-GFP2-TT-hixC-RFP2-TT-hixC
ACCESSION   BBa_S03785
VERSION     BBa_S03785.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     misc_feature    35..35
                     /label=extra base (avoid frameshift)
     CDS             36..248
                     /label=Continue coding sequence
     misc_feature    249..253
                     /label=(two in tandem)
     CDS             35..254
                     /label=BBa_I715023
     misc_feature    267..270
                     /label=conserved
     RBS             263..274
                     /label=BBa_B0034
     misc_feature    281..283
                     /label=Start Codon
     CDS             281..751
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    281..751
                     /label=BBa_I715019
     misc_feature    760..785
                     /label=BBa_J44000
     misc_feature    794..794
                     /label=extra base to maintain reading frame
     CDS             794..1037
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    1038..1043
                     /label=Stop Codons (two in tandem)
     misc_feature    794..1043
                     /label=BBa_I715020
     stem_loop       1063..1106
                     /label=stem_loop
     misc_feature    1052..1131
                     /label=BBa_B0010
     terminator      1052..1131
                     /label=BBa_B0010
     stem_loop       1147..1166
                     /label=T7 TE
     misc_feature    1173..1173
                     /label=stop
     polyA_site      1167..1180
                     /label=polya
     misc_feature    1140..1180
                     /label=BBa_B0012
     terminator      1140..1180
                     /label=BBa_B0012
     misc_feature    1189..1214
                     /label=BBa_J44000
     misc_feature    1223..1223
                     /label=extra base (avoid frameshift)
     CDS             1224..1436
                     /label=Continue coding sequence
     misc_feature    1437..1441
                     /label=(two in tandem)
     CDS             1223..1442
                     /label=BBa_I715023
     stem_loop       1462..1505
                     /label=stem_loop
     misc_feature    1451..1530
                     /label=BBa_B0010
     terminator      1451..1530
                     /label=BBa_B0010
     stem_loop       1546..1565
                     /label=T7 TE
     misc_feature    1572..1572
                     /label=stop
     polyA_site      1566..1579
                     /label=polya
     misc_feature    1539..1579
                     /label=BBa_B0012
     terminator      1539..1579
                     /label=BBa_B0012
     misc_feature    1588..1613
                     /label=BBa_J44000
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagtggtgc tctgaaaggt gaaatcaaaa
       61 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
      121 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
      181 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
      241 ccggtgctta ataatactag agaaagagga gaaatactag atgcgtaaag gagaagaact
      301 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      361 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      421 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      481 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      541 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      601 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      721 acacaatgta tacatcatgg cagacaaaca atactagagt tatcaaaaac catggttttt
      781 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      961 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1021 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1081 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1141 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt atcaaaaacc
     1201 atggtttttg ataatactag agtggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1261 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1321 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1381 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1441 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1501 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1561 gggcctttct gcgtttatat actagagtta tcaaaaacca tggtttttga taa
//