LOCUS       BBa_S03817              1902 bp    DNA     linear       19-May-2024
DEFINITION  I5010:I6036
ACCESSION   BBa_S03817
VERSION     BBa_S03817.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..856
                     /label=BBa_C0051
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     stem_loop       926..948
                     /label=stem_loop
     misc_feature    914..959
                     /label=BBa_B0011
     terminator      914..959
                     /label=BBa_B0011
     misc_feature    1..959
                     /label=BBa_I5010
     promoter        1005..1010
                     /label=-10
     promoter        982..987
                     /label=-35
     misc_feature    992..1008
                     /label=OR1
     misc_feature    968..984
                     /label=OR2
     misc_feature    968..1016
                     /label=BBa_R0051
     promoter        968..1016
                     /label=BBa_R0051
     misc_feature    1029..1032
                     /label=conserved
     RBS             1025..1036
                     /label=BBa_B0034
     CDS             1043..1765
                     /label=BBa_E0030
     stem_loop       1785..1828
                     /label=stem_loop
     misc_feature    1774..1853
                     /label=BBa_B0010
     terminator      1774..1853
                     /label=BBa_B0010
     stem_loop       1869..1888
                     /label=T7 TE
     misc_feature    1895..1895
                     /label=stop
     polyA_site      1889..1902
                     /label=polya
     misc_feature    1862..1902
                     /label=BBa_B0012
     terminator      1862..1902
                     /label=BBa_B0012
     misc_feature    968..1902
                     /label=BBa_I6036
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttt
      961 actagagtaa caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact
     1021 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt
     1081 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1141 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1201 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
     1261 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta
     1321 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1381 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1441 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1501 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1561 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1621 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     1681 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1741 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1801 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1861 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//