LOCUS       BBa_S03824              1876 bp    DNA     linear       18-May-2024
DEFINITION  S03823:R0071
ACCESSION   BBa_S03824
VERSION     BBa_S03824.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..761
                     /label=BBa_E1010
     stem_loop       806..849
                     /label=stem_loop
     misc_feature    795..874
                     /label=BBa_B0010
     terminator      795..874
                     /label=BBa_B0010
     stem_loop       890..909
                     /label=T7 TE
     misc_feature    916..916
                     /label=stop
     polyA_site      910..923
                     /label=polya
     misc_feature    883..923
                     /label=BBa_B0012
     terminator      883..923
                     /label=BBa_B0012
     misc_feature    1..923
                     /label=BBa_I13521
     misc_feature    936..939
                     /label=conserved
     RBS             932..943
                     /label=BBa_B0034
     CDS             950..1678
                     /label=BBa_C0171
     stem_loop       1698..1741
                     /label=stem_loop
     misc_feature    1687..1766
                     /label=BBa_B0010
     terminator      1687..1766
                     /label=BBa_B0010
     stem_loop       1782..1801
                     /label=T7 TE
     misc_feature    1808..1808
                     /label=stop
     polyA_site      1802..1815
                     /label=polya
     misc_feature    1775..1815
                     /label=BBa_B0012
     terminator      1775..1815
                     /label=BBa_B0012
     misc_feature    932..1815
                     /label=BBa_I1466
     misc_feature    1..1815
                     /label=BBa_S03823
     misc_feature    1824..1843
                     /label=RhlR
     promoter        1842..1847
                     /label=-35
     promoter        1865..1870
                     /label=-10
     misc_feature    1876..1876
                     /label=start
     promoter        1824..1876
                     /label=BBa_R0071
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atatactaga gaaagaggag aaatactaga tgaggaatga
      961 cggaggcttt ttgctgtggt gggacggttt gcgtagcgag atgcagccga tccacgacag
     1021 ccagggcgtg ttcgccgtcc tggaaaagga agtgcggcgc ctgggcttcg attactacgc
     1081 ctatggcgtg cgccacacga ttcccttcac ccggccgaag accgaggtcc atggcaccta
     1141 tcccaaggcc tggctggagc gataccagat gcagaactac ggggccgtgg atccggcgat
     1201 cctcaacggc ctgcgctcct cggaaatggt ggtctggagc gacagcctgt tcgaccagag
     1261 ccggatgctc tggaacgagg ctcgcgattg gggcctctgt gtcggcgcga ccttgccgat
     1321 ccgcgcgccg aacaatttgc tcagcgtgct ttccgtggcg cgcgaccagc agaacatctc
     1381 cagcttcgag cgcgaggaaa tccgcctgcg gctgcgttgc atgatcgagt tgctgaccca
     1441 gaagctgacc gacctggagc atccgatgct gatgtccaac ccggtctgcc tgagccatcg
     1501 cgaacgcgag atcctgcaat ggaccgccga cggcaagagt tccggggaaa tcgccatcat
     1561 cctgagcatc tccgagagca cggtgaactt ccaccacaag aacatccaga agaagttcga
     1621 cgcgccgaac aagacgctgg ctgccgccta cgccgcggcg ctgggtctca tctaataata
     1681 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1741 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1801 ctttctgcgt ttatatacta gagtcctgtg aaatctggca gttaccgtta gctttcgaat
     1861 tggctaaaaa gtgttc
//