LOCUS       BBa_S03825              1885 bp    DNA     linear       17-May-2024
DEFINITION  A340620:E0840
ACCESSION   BBa_S03825
VERSION     BBa_S03825.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     misc_feature    945..964
                     /label=LuxR/HSL
     promoter        964..969
                     /label=-35
     promoter        986..991
                     /label=-10
     misc_feature    997..997
                     /label=start
     misc_feature    945..999
                     /label=BBa_R0062
     promoter        945..999
                     /label=BBa_R0062
     misc_feature    1..999
                     /label=BBa_A340620
     RBS             1008..1022
                     /label=RBS-1\Strong
     RBS             1015..1019
                     /label=RBS
     misc_feature    1008..1022
                     /label=BBa_B0030
     RBS             1008..1022
                     /label=BBa_B0030
     CDS             1029..1748
                     /label=GFP protein
     CDS             1029..1748
                     /label=BBa_E0040
     stem_loop       1768..1811
                     /label=stem_loop
     misc_feature    1757..1836
                     /label=BBa_B0010
     terminator      1757..1836
                     /label=BBa_B0010
     stem_loop       1852..1871
                     /label=T7 TE
     misc_feature    1878..1878
                     /label=stop
     polyA_site      1872..1885
                     /label=polya
     misc_feature    1845..1885
                     /label=BBa_B0012
     terminator      1845..1885
                     /label=BBa_B0012
     misc_feature    1008..1885
                     /label=BBa_E0840
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac
      961 aggtttacgc aagaaaatgg tttgttatag tcgaataaat actagagatt aaagaggaga
     1021 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1081 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1141 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1201 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1261 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1321 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1381 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1441 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1501 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1561 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1621 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1681 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1741 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1801 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1861 tcgggtgggc ctttctgcgt ttata
//