LOCUS BBa_S03838 5461 bp DNA linear 01-May-2024 DEFINITION I746107:I746201 ACCESSION BBa_S03838 VERSION BBa_S03838.1 FEATURES Location/Qualifiers RBS 1..12 /label=B0034-sequence RBS RBS 1273..1284 /label=B0034-sequence RBS misc_feature 1267..1272 /label=BamHI site CDS 19..1266 /label=agrC coding sequence CDS 1291..1920 /label=agrA coding sequence misc_feature 1..1920 /label=BBa_I746100 stem_loop 1940..1983 /label=stem_loop misc_feature 1929..2008 /label=BBa_B0010 terminator 1929..2008 /label=BBa_B0010 stem_loop 2024..2043 /label=T7 TE misc_feature 2050..2050 /label=stop polyA_site 2044..2057 /label=polya misc_feature 2017..2057 /label=BBa_B0012 terminator 2017..2057 /label=BBa_B0012 promoter 2066..2161 /label=BBa_I746104 RBS 2170..2184 /label=RBS-1\Strong RBS 2177..2181 /label=RBS misc_feature 2170..2184 /label=BBa_B0030 RBS 2170..2184 /label=BBa_B0030 CDS 2191..2910 /label=GFP protein CDS 2191..2910 /label=BBa_E0040 stem_loop 2930..2973 /label=stem_loop misc_feature 2919..2998 /label=BBa_B0010 terminator 2919..2998 /label=BBa_B0010 stem_loop 3014..3033 /label=T7 TE misc_feature 3040..3040 /label=stop polyA_site 3034..3047 /label=polya misc_feature 3007..3047 /label=BBa_B0012 terminator 3007..3047 /label=BBa_B0012 promoter 3056..3090 /label=BBa_J23111 misc_feature 3103..3106 /label=conserved RBS 3099..3110 /label=BBa_B0034 CDS 3117..5324 /label=BBa_I746200 stem_loop 5344..5387 /label=stem_loop misc_feature 5333..5412 /label=BBa_B0010 terminator 5333..5412 /label=BBa_B0010 stem_loop 5428..5447 /label=T7 TE misc_feature 5454..5454 /label=stop polyA_site 5448..5461 /label=polya misc_feature 5421..5461 /label=BBa_B0012 terminator 5421..5461 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gattctgatg ttcaccattc cagcaattat tagcggtatt 61 aagtacagca agctggatta tttcttcatc atcgtaatct ctaccctgtc cctgttcctg 121 ttcaaaatgt tcgattccgc gagcctgatt atcctgactt ccttcatcat catcatgtat 181 ttcgtaaaaa tcaaatggta tagcatcctg ctgatcatga cctcccagat tatcctgtac 241 tgtgccaact acatgtatat cgttatctac gcgtacatca cgaaaatctc cgattctatc 301 ttcgttatct ttccgtcttt cttcgtggtg tacgttacga tctctattct gttttcctat 361 attatcaatc gcgttctgaa aaagatctct actccgtacc tgattctgaa caaaggtttt 421 ctgatcgtca tctccactat cctgctgctg accttctctc tgttcttctt ttactctcag 481 atcaactccg atgaggcaaa agtcatccgt cagtacagct tcatctttat cggtattact 541 atcttcctga gcattctgac tttcgttatc agccagttcc tgctgaaaga aatgaagtac 601 aagcgcaacc aagaagaaat cgagacttac tacgagtaca ccctgaagat tgaagcgatc 661 aacaacgaaa tgcgtaaatt tcgtcacgac tacgttaaca ttctgaccac cctgagcgaa 721 tacatccgtg aagacgacat gcctggcctg cgtgattatt tcaacaaaaa cattgtgccg 781 atgaaagata acctgcaaat gaacgctatt aagctgaacg gtatcgaaaa cctgaaagta 841 cgtgagatca agggtctgat taccgctaaa atcctgcgtg cgcaggaaat gaacatccca 901 attagcatcg aaatcccaga cgaagtttct agcattaacc tgaacatgat tgacctgtct 961 cgtagcatcg gcatcatcct ggacaacgct atcgaagctt ccactgaaat cgacgacccg 1021 attattcgcg ttgcttttat cgagagcgaa aactccgtga cctttatcgt gatgaacaaa 1081 tgcgcggacg atatcccgcg tattcacgaa ctgttccagg aatctttcag caccaagggt 1141 gaaggtcgcg gcctgggcct gtccaccctg aaagagatcg cggataacgc cgacaacgtt 1201 ctgctggaca ctattatcga aaacggtttc ttcattcaga aagtcgaaat catcaataac 1261 taataaggat ccaaagagga gaaatactag atggaaatcg cactggctac ggacaatcca 1321 tatgaggttc tggaacaggc taaaaacatg aacgacatcg gctgctattt cctggatatt 1381 cagctgagca ccgacatcaa cggtatcaaa ctgggctctg aaatccgtaa acatgatccg 1441 gttggtaaca ttattttcgt gacttctcat tctgagctga cctatctgac gttcgtatac 1501 aaggttgcag ccatggattt catctttaaa gacgatccgg cagagctgcg tacgcgcatc 1561 atcgactgtc tggaaactgc gcacacgcgc ctgcaactgc tgtccaaaga taactccgtg 1621 gaaaccattg aactgaaacg tggcagcaac agcgtttacg ttcagtacga tgatatcatg 1681 ttcttcgaaa gctccactaa gtcccaccgt ctgatcgcgc atctggataa tcgtcagatc 1741 gagttctatg gcaacctgaa agagctgagc cagctggacg atcgcttctt ccgttgtcat 1801 aactccttcg ttgttaaccg tcacaacatc gaatccatcg actccaaaga gcgtattgta 1861 tacttcaaga acaaagaaca ctgttatgcg tctgtacgca atgtcaagaa aatctaataa 1921 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1981 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2041 gcctttctgc gtttatatac tagagattaa atacaaatta catttaacag ttaagtattt 2101 atttcctaca gttaggcaat ataatgataa aagattgtac taaatcgtat aatgacagtg 2161 atactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga 2221 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 2281 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 2341 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc 2401 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa 2461 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 2521 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 2581 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 2641 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 2701 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 2761 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 2821 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 2881 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa 2941 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3001 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttgac 3061 ggctagctca gtcctaggta tagtgctagc tactagagaa agaggagaaa tactagatga 3121 acaagaagat tcattccctg gccttgttgg tcaatctggg gatttatggg gtagcgcagg 3181 cacaagagcc gaccgatact cctgtttcac atgacgatac tattgtcgtt accgccgccg 3241 agcagaactt acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc 3301 cggttgcccg cgatgtgtcg aagatcatcc gtaccatgcc aggcgttaac ctgaccggta 3361 actccaccag tggtcagcgt gggaataacc gacagattga tattcgcggt atgggtccgg 3421 aaaacacgct gattttgatt gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg 3481 gctggcgtgg cgagcgcgat acccgtggtg atacttcctg ggtgccacct gaaatgattg 3541 aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg 3601 gcgtggttaa catcattacc aaaaaaggca gcggcgagtg gcacggctcc tgggacgcat 3661 atttcaatgc gccagaacat aaagaggaag gtgccaccaa acgcactaac tttagcctga 3721 ccggtccgct gggcgacgag ttcagcttcc gtttgtatgg caacctcgac aaaacccagg 3781 ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg tatgccacga 3841 cgttaccagc cgggcgcgaa ggggtaatca acaaagatat taatggcgtg gtgcgctggg 3901 atttcgcgcc attgcaatcg ctggaactgg aagcaggtta cagccgccag ggtaacctgt 3961 atgcgggcga cacccagaat accaactccg attcctatac ccgctcgaaa tatggcgatg 4021 aaaccaaccg tctgtatcgc cagaactacg cgctgacctg gaacggtggc tgggataacg 4081 gcgtgaccac cagcaactgg gtgcagtacg aacacacccg taactcgcgt attccggaag 4141 gtctggcggg cggtaccgaa gggaaattta acgaaaaagc gacacaggat ttcgtcgata 4201 tcgatcttga tgacgtgatg ctgcacagcg aagttaacct gccgattgat ttcctcgtta 4261 accagacgct gacgctgggt acggagtgga atcagcaacg gatgaaggac ttaagttcca 4321 acacccaggc actgaccgga acgaataccg gtggcgctat tgatggcgtg agtaccaccg 4381 accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac aacatggagc 4441 tgactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt attgtcggca 4501 ataactggag cccggcgctg aacatatcgc aaggtttagg cgatgacttc acgctgaaaa 4561 tgggcatcgc ccgtgcttat aaagcgccga gcctgtacca gactaacccg aactacattc 4621 tctacagtaa aggtcagggt tgctatgcca gcgcgggcgg ctgctatctg caaggtaacg 4681 atgacctgaa agcagaaacc agcatcaaca aagagattgg tctggagttc aaacgcgacg 4741 ggtggctggc gggcgtcacc tggttccgta acgattatcg caataagatt gaagcgggct 4801 atgtggctgt agggcaaaat tgtgaaacag cggtggttga aggtctggaa ggatcgttaa 4861 acgtaccggt tagcgaaacg gtgatgtgga ccaataacat cacttatatg ctgaagagtg 4921 aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtatacgttg aactcaacgc 4981 tgagctggca ggcacgggaa gatttgtcga tgcaaacgac cttcacctgg tacggcaagc 5041 agcagccgaa gaagtacaac tataaaggtc agccagcggt tggaccggaa accaaagaaa 5101 ttagtcctta cagcattgtt ggcctgagcg cgacctggga tgtgacgaag aatgtcagtc 5161 tgaccggcgg cgtggacaat ctgttcgaca aacgtttgtg gcgtgcgggt aatgcccaga 5221 ccacgggcga tttggcaggg gccaactata tcgccggtgc cggggcgtat acctataacg 5281 agccgggacg tacgtggtat atgagcgtaa acacccactt ctgatactag agccaggcat 5341 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 5401 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 5461 a //