LOCUS       BBa_S04062              1076 bp    DNA     linear       27-May-2024
DEFINITION  K137046:S03998
ACCESSION   BBa_S04062
VERSION     BBa_S04062.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=tetR promoter
     promoter        1..150
                     /label=BBa_K137046
     misc_feature    159..175
                     /label=IRR out
     misc_feature    177..193
                     /label=IRR in
     misc_feature    159..193
                     /label=BBa_K137008
     misc_feature    206..209
                     /label=conserved
     RBS             202..213
                     /label=BBa_B0034
     CDS             220..939
                     /label=GFP protein
     CDS             220..939
                     /label=BBa_E0040
     stem_loop       959..1002
                     /label=stem_loop
     misc_feature    948..1027
                     /label=BBa_B0010
     terminator      948..1027
                     /label=BBa_B0010
     stem_loop       1043..1062
                     /label=T7 TE
     misc_feature    1069..1069
                     /label=stop
     polyA_site      1063..1076
                     /label=polya
     misc_feature    1036..1076
                     /label=BBa_B0012
     terminator      1036..1076
                     /label=BBa_B0012
ORIGIN
        1 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactctag
       61 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      121 caccgacaaa caacagataa aacgaaaggc tactagagaa gatgaaacat ttggggccaa
      181 actgtccata ttatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt
      241 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      301 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      361 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      421 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      481 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      541 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      601 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      661 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      721 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
      781 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
      841 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
      901 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
      961 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1021 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//