LOCUS       BBa_S04063              1176 bp    DNA     linear       19-May-2024
DEFINITION  K137047:S03998
ACCESSION   BBa_S04063
VERSION     BBa_S04063.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=tetR promoter
     promoter        1..250
                     /label=BBa_K137047
     misc_feature    259..275
                     /label=IRR out
     misc_feature    277..293
                     /label=IRR in
     misc_feature    259..293
                     /label=BBa_K137008
     misc_feature    306..309
                     /label=conserved
     RBS             302..313
                     /label=BBa_B0034
     CDS             320..1039
                     /label=GFP protein
     CDS             320..1039
                     /label=BBa_E0040
     stem_loop       1059..1102
                     /label=stem_loop
     misc_feature    1048..1127
                     /label=BBa_B0010
     terminator      1048..1127
                     /label=BBa_B0010
     stem_loop       1143..1162
                     /label=T7 TE
     misc_feature    1169..1169
                     /label=stop
     polyA_site      1163..1176
                     /label=polya
     misc_feature    1136..1176
                     /label=BBa_B0012
     terminator      1136..1176
                     /label=BBa_B0012
ORIGIN
        1 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactctag
       61 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      181 ttgatgcctg gctctagtag tgatctacac tagcactatc agtgttatta agctactaaa
      241 gcgtagtttt tactagagaa gatgaaacat ttggggccaa actgtccata ttatactaga
      301 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      361 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      421 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      481 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      541 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      601 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      661 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      721 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      781 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      841 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      901 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      961 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1021 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1081 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1141 ctggctcacc ttcgggtggg cctttctgcg tttata
//